摘要
目的：为研究ＨＳＶ－ｔｋ／ＧＣＶ系统对涎腺腺样囊性癌细胞株（ＡＣＣ－Ｍ）细胞的体外杀伤作用。方法：采用ＭＴＴ法和细胞计数法对ＧＣＶ在不同剂量和不同作用时间时细胞的生存率进行研究。结果：ＨＳＶ－ｔｋ／ＧＣＶ系统能有效地杀伤ＡＣＣ－Ｍ细胞，杀伤呈时间依赖性和剂量依赖性，这种作用有比较明显的旁杀伤效应，旁杀伤效应的强弱与细胞间的接触程度呈正比关系。结论：ＨＳＶ－ｔｋ／ＧＣＶ系统能有效地杀伤ＡＣＣ细胞，可望用于ＡＣＣ的治疗。
Objective: To study the effects of HSV tk/GCV system on adenoid cystic carcinoma(ACC) cells in vitro. Methods: Adenoid cystic carcinoma cells transduced HSV tk gene were treated with ganciclovir(GCV) in different concentrations and at different times, the cell killing was detected with MTT assay. Results: HSV tk/GCV system can kill the ACC M cells greatly in dose dependent and time dependent way, there are apparent bystander effect among cell killing. Conclusions: The HSV tk/GCV system can be used to treat ACC.
出处
《口腔颌面外科杂志》
CAS
1999年第1期26-29,共4页
Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金
