摘要
目的探讨大肠癌中多药耐药基因ＭＤＲ１与ｐ５３表达之间的关系。方法利用免疫组化研究６０例大肠癌及２０例大肠腺瘤ＭＤＲ－１，ｐ５３的表达。结果显示６０例大肠癌切片中ＭＤＲ－１与ｐ５３的阳性表达率为５７％和３７％，１０例正常大肠组织和２０例大肠腺瘤仅２０％显示ＭＤＲ－１阳性表达，ｐ５３在正常大肠组织，管状腺瘤中全部阴性，仅一例有不典型增生的绒毛状腺瘤为阳性表达。同时显示ｐ５３在不同分化的大肠癌中与ＭＤＲ表达呈正相关。结论１）通过检测ＤＭＲ－１可以对大肠癌病人进行化疗敏感性预测。２）ｐ５３过度表达对ＭＤＲ－１基因可能具有诱导和增加效应。３）ｐ５３和ＭＤＲ－１一起可用作判断病人预后，生物学行为的一个有用参数。
PURPOSE To investigate the relationship between multidrug resistance gene and p53 protein in large intestinal carcinoma.METHODS Microwave immunohistochemical staining was used to detect MDR -1 and p53 in 60 cases of large intestinal carcinoma and 20 cases of adenoma.RESULTS The positive rate of MDR -1 and p53 was 57% and 37% respectively in 60 carcinomas,while in 10 normal large intestinal tissue and 20 adenomas,only 20% were positive for MDR -1 . All the tubular adenomas were negative and only one villous adenoma dysplasia was positive for p53.The expression of p53 was positively correlated with MDR -1 in various differentiated large intestinal carcinoma.CONCLUSION 1)Overexpression of p53 protein might have inducing and increasing effects on MDR -1 gene expression.2) MDR -1 provides us a useful marker to predict chemotherapy sensitivity.3)p53 combined with MDR -1 can be an important parameter in prediction of the prognosis and biological behavior of large intestinal carcinoma patients.
出处
《中国癌症杂志》
CAS
CSCD
1999年第1期47-48,共2页
China Oncology
关键词
大肠癌
免疫组化
多药耐药基因
P53基因表达
Large intestinal carcinoma Immunohistochemical staining Multidrug resistance gene p53
