摘要
目的:对中药天麻中的一种葡聚糖GBⅡ进行研究。方法:天麻药材经过水提醇沉得到天麻粗多糖,经DEAE-Sepharose Fast Flow离子交换色谱柱层析和Sepharose CL-6B纯化得到均一多糖GBⅡ,经13C-NMR、IR谱、单糖组分分析、甲基化分析等方法确定其结构。结果:GBⅡ相对分子量为4300,由27个葡萄糖组成的葡聚糖,以α-D-1,4连接的葡萄糖构成主链,在葡萄糖6位带有少量分支;其中尚含有极少量葡萄糖醛酸。结论:GBⅡ为从天麻中首次分离得到的均一多糖。
Objective：To study a glucan（GBⅡ）isolated from Gastrodia elata.Methods：The glucan was obtained with water extraction,alcohol precipitate,DEAE-Sepharose Fast Flow column and Sepharose CL-6B column chromatography;sugar composition analysis,IR and NMR were used to determine the structural feature.Results：The molecular weight of the glucan was 4 300 dalton estimated by HPGPC;it contained 27 glucose residues,which mainly composed of α-D-（1→4）-glucose with little glucuronic acid and branch O-6 points.Conclusion：The glucan was a new glucan for the first report from Gastrodia elata.
出处
《中药材》
CAS
CSCD
北大核心
2010年第5期726-729,共4页
Journal of Chinese Medicinal Materials
基金
中药药效物质基础及物质资源库研究关键技术(2009ZX09502-013)
关键词
天麻
葡聚糖
结构鉴定
Gastrodia elata Bl.
Glucan
Structural characterization
