摘要
目的探讨放射免疫介导再障小鼠骨髓中转录因子T-bet的表达及其意义。方法建立再障小鼠模型，取再障和正常组小鼠骨髓单个核细胞（BMMNC），在终浓度为15拉g／mL植物血凝素（PHA）条件下培养，用双抗体夹心ELISA方法检测小鼠骨髓淋巴细胞培养上清Thl及Th2类细胞因子IL-2、IFN-γ和IL．4、IL．10的浓度水平；用RT-PCR技术检测小鼠骨髓转录因子T-betmRNA表达强度。结果再障小鼠骨髓BMMNC培养上清中细胞因子IL-2、IFN-γ水平均显著高于正常组（P〈0．01），而IL．4、IL-10水平均明显低于正常组（P〈0．01）；转录因子T-betmRNA表达也明冠高于正常组（P〈0．01）。结论T-bet表达增强可能是再障小鼠出现Th1细胞优势分化状态的重要原因之一。
Objective To investigate the transcription factor T-bet in bone marrow in immune mediated aplastic anemia（AA） mice. Methods Established model of AA mice. Mononuclear cells were isolated from bone marrow incubated with phytohemagglutinin （PHA）（1 5 μg/mL） in vitro.IL-2 and IFN- γ in cultured supernatant were detected by EL1SA.The expression level of transcription factor T-bet was assayed by RT-PCR. Results In AA mice,the level of IL-2 and IFN- γ were much higher than normal （P〈0.01 ）,but IL-4,IL- 10 were lower than normal（P〈0.0 1）.And the expression of T-bet mRNA was also significantly higher than normal. Conclusion Shifted development of Th toward to Thl in AA mice,and T-bet significantly over expression may be the most important reason.
出处
《中国血液流变学杂志》
CAS
2010年第2期178-180,共3页
Chinese Journal of Hemorheology
