摘要
目的 拟建立大鼠急性视神经钳夹损伤模型,在大鼠视神经损伤后将不同的剂量的neuregulin-1（NRG-1）注入玻璃体腔内,研究NRG-1是否对视神经急性损伤有保护和修复作用,并以此推测视神经急性损伤的可能机制.方法 2月龄SD雄性大鼠100只.随机分为NRG-1治疗组、生理盐水对照组、阴性对照组;然后于1、2、7、14、28d各组行FVEP检查及眼底照相定性观察视神经受损及修复情况并分别处死部分动物,用组织病理检查、TUNEL原位凋亡检测等技术来观察和评价视网膜神经节细胞的数目及进行各组间视网膜神经节细胞凋亡数目的 比较.结果 从眼底照相、FVEP、HE染色、TUNEL原位凋亡检测等方面进行评估.结论 NRG-1能有效降低视网膜组织细胞的凋亡数目,并且其保护作用的效果可能与剂量有关.这些结果说明：大鼠急性视神经损伤后,给予NRG-1能降低视网膜神经节细胞的凋亡,说明NRG-1对于急性视神经损伤具有修复和保护作用.视神经损伤后,尽量保存视网膜神经节细胞免于凋亡是改善视神经损伤预后的关键.我们的研究结果提示：尽早使用外源性NRG-1等神经保护剂,保护RGCs等神经元,对于损伤后神经再生、进而神经功能的恢复可能有积极作用.
Objective This research set up the model of acute optic nerve ring clamp injury,And inject variation dosage of NRG-1 in vitreous chamber,to study the possible mechanism and the interaction among them.Methods Disregarding gender,100 matured rats were medially divided into 5 groups randomly.common group （n=20）,acute optic nerve wound group;all left eye of experimental animal for operation,right eye do nothing,to build model as part 1,after injury neuregulin-1 0.5ug,1ug,3ug（each group n=20） were injcoted into vitreous chamber one time respectively,isometrical NS were injected in the control group （n=20）.After ring clamp 1d,2d,7d,14d,and 28d every group professed FVEP and fundus photography to qualitation optic nerve damage and reparation.1d,2d,7d,14d,and 28d after operation,experimental animal was put to death,eyeballs involving ON were obtained tO evaluate the damage using light microscope after HE staining and TUNEL to analyze the changes of RGCs and its apoptosis compared with the control group in vitro after execution.Results Morphous,electrophysiology,pathology observation after building model.The expression of NRG-1：Compared to sham operated group,2h,1d,2d,7d,14dafter acute ON injury,The expression of NRG-1 at each time point was obviously increasing（P 〈0.05）,among the total,14d was peak;then fall-off to normal in 28d,which was not statistically significant to sham operated group （P〉0.05）.Conclusions NRG-1 can effectively reduce the apoptosis of nerve cell and retina tissue,this result suggests that NRG-1 may play essential role in repairing and protecting acute optic nerve injury.It is critical to reduce the damage of optic nerve injury and protect RGCs from apoptosis. Based on our results,it is better to apply NRG-1 as early as possible after acute optic nerve injury.
出处
《中国实用眼科杂志》
CSCD
北大核心
2010年第7期789-793,共5页
Chinese Journal of Practical Ophthalmology
