摘要
目的:探讨缬沙坦对尼古丁所致大鼠肠系膜动脉内皮依赖性舒张(EDR)功能损伤的保护作用及其机制。方法:将30只大鼠分为5组,即正常对照、尼古丁损伤组(2mg/kg,腹腔注射)、缬沙坦高、中、低剂量组(尼古丁2mg/kg,腹腔注射+缬沙坦30、10、3mg/kg,灌胃),6周后检测各组肠系膜动脉环EDR及血清一氧化氮(NO)含量、一氧化氮合成酶(NOS)、丙二醛含量(MDA)、超氧化物歧化酶(SOD)活性变化。结果:尼古丁使肠系膜动脉环EDR明显降低(54.03%),并伴随血清NO含量及NOS、SOD活性的下降及MDA含量的升高;而缬沙坦(中、高剂量组)使其EDR得到明显改善(73.06%、82.95%),并且抑制了尼古丁诱导的NO含量,NOS、SOD活性的下降及MDA的升高(P<0.05,与尼古丁损伤组比较)。结论:缬沙坦对尼古丁所致的血管内皮损伤具有明显保护作用,该作用可能与其抗氧化,促进内皮细胞合成、释放NO有关。
Objective To explore the protective effects and mechanism of valsartan on the endothelium- dependent relaxation （EDR） of nicotine-induced impairment in rats mesenteric arteries. Methods Thirty rats were divided into 5 groups randomly as control group, nicotine injuried group （2 mg/kg,ip）, and valsartan high-dose group, valsartan middle-dose group and valsartan low-dose group, （30 mg/kg, 10 mg/kg or 3 mg/kg, ig）. The EDR responses of mesenteric arteries rings and the serum contents of nitric oxide （NO） , nitric oxide synthase （NOS）, superoxide dismutase（SOD）, malondialdehyde （MDA） activities were detected after 6 weeks. Results Nicotine significantly inhibited EDR responses of mesenteric arteries rings （54.03% ）. Nicotine decreased NO, NOS, SOD activities and increased MDA activities in serum. While in valsartan high-dose group and valsartan middle-dose group, the EDR responses （73.06, 82.95% ） improved significantly. Serum NOS, SOD activities decreased and MDA activities increased（P 0.05, compared to nicotine injuried group）. Conclusion Valsartan can be used to protect nicotine-induced endothelial dysfunction.It may be related to its antioxidation, promoting the synthesis of endothelial and releasing NO.
出处
《实用医学杂志》
CAS
北大核心
2010年第12期2123-2125,共3页
The Journal of Practical Medicine
关键词
尼古丁
缬沙坦
内皮依赖性舒张
Nicotine
Valsartan
Endothelium-dependent relaxation
