摘要
通过对中国二化螟(Chilo suppressalis Walker)14个地理种群的线粒体DNACOⅡ基因和核糖体DNAITS基因的测序,分析了二化螟不同地理种群之间的COⅡ和ITS序列的进化分歧及相似性;同时运用Mega 3.0软件建立其系统发育关系。结果表明:14个地理种群间的COⅡ基因共有8个变异位点,占全长的1.4%,而且这些变异位点全部发生在密码子第3位点,江西宁都种群(ND)与其他13个种群的核苷酸差异相对较大,有5~7个碱基的差异,占全长的的0.88%~1.23%;ITS序列长度变异范围为545~585 bp,平均为576 bp,长度变异为40 bp。进化树显示,在COⅡ分子标记方法中江西宁都种群与其他种群差异最大,而在ITS分子标记方法中江西兴国种群与其他种群差异最大;两种方法中,龙南、婺源、庐江和上杭4个种群都聚为一支。
The cytochrome oxidase subunit Ⅱ(COⅡ) located in mitochondrial DNA(mtDNA) and internal transcribed spacers(ITS)located in ribosomal DNA(rDNA)among 14 geographical populations of Chilo suppressalis Walker in China were sequenced.The identity and divergent evolution of COⅡ and ITS sequences among populations were analyzed and the phylogenetic relationships were established by Mega 3.0.The results showed that the COⅡ sequence among different geographical populations of Chilo suppressalis had eight variable positions,which accounted for 1.4% of the total length.Fourthmore,all of these variations occurred at the third codon.There were 5-7 variable positions between Ningdu population(ND) and 13 other populations,which accounted for 0.88%-1.23% of the total length.The length of ITS was from 545 bp to 585 bp with variation of 40 bp,with the average length of 576 bp.The phylogenetic trees based on COⅡ sequence revealed that there were the most diversity between Ningdu population(ND) and 13 other populations.However,the phylogenetic trees based on ITS sequence showed that there were the most diversity between Xingguo population(XG) and 13 other populations.Longnan(LN),Wuyuan(WY),Lujiang(LJ) and Shanghang(SH) populations were in one clade revealed by the phylogenetic trees based on both COⅡ sequence and ITS sequence.
出处
《江苏农业学报》
CSCD
北大核心
2010年第3期493-498,共6页
Jiangsu Journal of Agricultural Sciences
基金
现代农业产业技术体系建设专项基金(011032310813)
公益性行业(农业)科研专项(200803004)
关键词
二化螟
COⅡ基因
ITS基因
地理种群
系统发育关系
Chilo suppressalis
cytochrome oxidase subunit Ⅱ
internal transcribed spacer
geographical population
phylogenetic relationship