摘要
研究了1株中度嗜盐异养硝化菌gs2的脱氮特性和耐盐性能。从处理高盐度废水的成熟活性污泥中分离出1株中度嗜盐的异养硝化细菌gs2,对该菌株进行形态观察、生理生化试验以及16S rDNA序列同源性分析。结果表明,该菌株为盐单胞菌属(Halomonas),能在NaCl质量分数为0～20%的培养液中生长;能耐受较宽范围的pH值(6～11),且最适pH值为8;在25～35℃下均可生长,且30℃时生长最佳。采用CH_3COONa和NH_4Cl作为碳源和氮源对菌株gs2进行硝化特性研究,经过24 h培养后gs2对NH_4^+～N和COD的最终去除率分别为81%和74.24%;在亚硝化和反硝化培养基中经过24 h后,gs2对NO_2^--N和NO_3^--N的最终去除率均可达到90%以上,表明该菌株可实现同步硝化和反硝化,即可以独立完成生物脱氮的全部过程。
Nitrogen removal characteristics and halotolerancy of strain gs2 were studied. The present paper is inclined to introduce our work on identifying a moderately halophilic nitrifying bacterium and its experimental application to high-salinity sewage treating process. As is known, a moderately halophilic nitrifying bacteria, short for gs2, can be screened from the activated sludge which can be used for treating high-salinity sewage. We have succeeded in isolating and identifying a strain of gs2, known as Halomonas sp, through our morphological observation, physiological-biochemical testing and the sequence analysis of 16S rDNA. In contrast to the most previously described heterotrophic nitrifying bacterium, the strain can grow under higher salinity （NaCl mass fraction of 0%-20% ）. It is also tolerant to a wide range of pH value （6 -11 ） and temperature （25 - 35 ℃ ）, with its optimal condition for growth being at 30 ℃ and pH = 8. In proceeding with our research, we have also investigated the nitrification characteristics of the bacterium. We have also found that through a 24 h aerobic culture, it is possible to make the removal rates of NH4^＋ N and COD by strain gs2 go up to 81% and 74.24% respectively. In addition, if nitrosifying culture medium and denitrifying culture medium were used, it is possible to make the removing rates of the NO2^- - N and NO3^- - N go over 90% after a 24 h aerobic culture with the help of strain gs2. Thus, it can be seen that this strain proves to be able to perform the whole process of biological nitrogen removal independently.
出处
《安全与环境学报》
CAS
CSCD
北大核心
2010年第3期13-17,共5页
Journal of Safety and Environment
基金
国家自然科学基金项目(50678085
50878107)
青岛大学优秀研究生学位论文培育项目(YSPY2009014)
关键词
环境工程学
异养硝化细菌
生物脱氮
好氧反硝化
中度嗜盐菌
environmental engineering
heterotrophic nitrifyingbacteria
biological nitrogen removal
aerobicdenitrification
moderately halophilic bacterium
