摘要
目的:对不同厂家药品微生物限度检查用细菌、真菌计数培养基的部分质量指标进行比较。方法:以市售的常见品牌及实验室自制的共7种营养琼脂和5种玫瑰红钠琼脂培养基为对象,测定其水分、含氮量、pH值等理化指标,并用5株代表菌株进行加菌回收计数,比较生长菌落数量及菌落大小。结果:不同厂家的培养基理化指标、加菌回收的菌落数量和菌落大小均有差异。结论:不同厂家培养基质量存在一定差异,培养基的理化指标与培养基质量有一定相关性。
OBJECTIVE：To compare the quality of fungus and bacteria counting culture medium of drug microbial limit test among different manufacturers.METHODS：Culture medium for 7 kinds of nutrient agars and 5 kinds of sodium rose red bengal agars for sale or self-made were enrolled in study.The content of water and nitrogen,pH value were determined.The number of microcolonies which were recovered from 5 kinds of representative bacterial strains was calculated,and the size and number of microcolonies were compared.RESULTS：Physicochemical index of culture medium,the size and number of microcolonies were different among different manufacturers.CONCLUSION：The quality of culture medium is different among different manufacturers,which is associated with physicochemical index.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第25期2365-2368,共4页
China Pharmacy
关键词
厂家
微生物限度检查
培养基
质量比较
Manufacturers
Microbial limit test
Culture medium
Quality comparison
