摘要
目的:研究人参皂甙Rg1对人慢性髓细胞白血病敏感株K562细胞p210bcr/abl融合蛋白表达的影响。方法:体外培养K562细胞,经人参皂甙Rg1处理不同时间后,用流式细胞仪检测细胞凋亡;用Western印迹技术检测细胞内p210bcr/abl融合蛋白表达。结果:人参皂甙Rg1可诱导细胞凋亡,凋亡率并随时间延长而升高;人参皂甙Rg1可下调p210bcr/abl蛋白表达量,并具时间依赖性。结论:人参皂甙Rg1可通过降低p210bcr/abl蛋白水平来诱导K562细胞凋亡,达到有效抗人慢性髓细胞白血病的效果。
Objective：To investigate the effects of ginsenoside Rg1 on the expressions of p210 bcr/abl fusion protein in human chronic myeloid leukemia（CML）.Methods：Human Ph＋ CML K562 cells were cultured in vitro and treated with ginsenoside Rg1 for different time.Flow cytometry（FCM） was used to evaluate the apoptosis of leukemic cells.The protein levels of p210 bcr/abl in the leukemic cells were determined by Western blotting.Results：Flow cytometry showed that ginsenoside Rg1 can induce the apoptosis of K562 cells,and the percentage of apoptotic cells increased with treating time extending.Western blotting showed that ginsenoside Rg1 down-regulated the expressions of p210 bcr/abl fusion protein in a time-dependent manner.Conclusion：Ginsenoside Rg1may induce the apoptosis of K562 cells to inhibit CML effectively through down-regulating the expressions of p210 bcr/abl fusion protein.
出处
《现代生物医学进展》
CAS
2010年第10期1851-1853,共3页
Progress in Modern Biomedicine
