摘要
将两个来源于草菇乙酰木聚糖酯酶的家族I的纤维素结合域(CBD)融合到一起形成重组蛋白,并将该功能性纤维素吸附重组蛋白在大肠杆菌中进行表达,利用该重组蛋白处理What-man滤纸后检测滤纸的机械性能。检测发现经纯化的重组蛋白处理后的滤纸其机械性能都有所提高,其中抗张强度提高了10%,耐破度提高了8.7%,耐折度提高了30%。该研究表明CBD在纤维及造纸工业等方面具有巨大的潜在应用价值。
Two family-I cellulose-binding domains（CBD） of acetyl xylan esterase from Volvariella volvacea were fused to form a recombinant protein.The recombinant bifunctional cellulose-binding protein was expressed in E.coli.Then the mechanical properties of Whatman filter paper treated with the recombinant protein were tested.The purified protein improved the treated paper′s tensile strength,bursting strength and folding endurance by 10%,8.7% and 30%,respectively.This research demonstrated that the cellulose-binding domains have an immense potential for the application in the field of fiber and paper industries.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2010年第3期61-65,共5页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(30671652)
南京林业大学科技创新基金(cx07-030-2)
关键词
乙酰木聚糖酯酶
纤维素结合域
机械性能
纸张增强剂
acetyl xylan esterase
cellulose-binding domain
mechanical properties
paper-strengthening agent
