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不同fimA基因型牙龈卟啉单胞菌黏附和侵入口腔上皮细胞能力的比较 被引量:1

Comparative study on the ability of adhesion and invasion of different fimA genetypes of Porphyromonas gingivalis to oral epithelial cells
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摘要 目的通过比较临床分离的不同fimA型牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)菌株黏附和侵入KB细胞的能力,探讨不同fimA基因型魄菌株之间致病力差异的原因。方法应用培养法和抗生素保护法测定不同fimA型Pg菌株黏附和侵入KB细胞的能力,扫描电镜观察不同fimA型Pg对KB细胞的黏附情况。结果所有菌株均可黏附和侵入KB细胞,黏附率为0.523%~37.125%,侵入率为0.017%~3.750%;各fimA型Pg菌株之间黏附和侵入KB细胞的能力差异无统计学意义(P〉0.05);各株Pg之间、4株Ⅱ型菌株之间、5株Ⅳ型菌株之间,黏附和侵入KB细胞的能力差异有统计学意义(P〈0.01)。结论Pg的fimA型与其对KB细胞的黏附和侵入能力无相关性,提示可能存在其他影响不同fimA型Pg致病力差异的因子。 Objective To compare the ability of adhesion and invasion to epithelial cells by Porphyromonas gingivalis (Pg) strains with different fimA separated from Chinese. Methods Cultured method and antibiotic protection method were used to determine the adhesive and invasive ability of Pg with different fimA genetypes. The adhesion was observed by scanning electron microscope. Results All the strains adhered and invaded to KB cells, and the adhesion rate ranged from 0. 523% to 37. 125% and invasive rate from 0. 017% to 3. 750%. The adhesive and invasive ability among different fimA genotypes showed no significant difference (P 〉 0. 05 ). Conclusions There is no significant correlation between fimA genotype and ability in adhesion and invasion to KB cells.
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2010年第6期342-345,共4页 Chinese Journal of Stomatology
基金 国家自然科学基金(30471890) 教育部高等学校博士学科点专项科研基金(20070610066)
关键词 紫单胞菌 上皮细胞 细胞黏附 firnA基因型 Porphyromonas gingivalis Epithelial cells Bacterial adhesion fimA genotype
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  • 1Fujiwara T,Nakagawa I,Morishima S,et al.Inconsistency between the fimbrilin gene and the antigenicity of lipopolysaccharides in selected strains of Porphyromonas gingivalis.FEMS Microbiol Lett,1994,124(3):333-341. 被引量:1
  • 2Nakagawa I,Amano A,Kimura RK,et al.Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of fimA gene.J Clin Microbiol,2000,38(5):1909-1914. 被引量:1
  • 3Nakagawa I,Amano A,Ohara-Nemoto Y,et aL Identification of a new variant of fimA gene of Porphyromonas gingivalis and its distribution in adults and disabled populations with periodontitis.J Periodontal Res,2002,37(6):425-432. 被引量:1
  • 4Nakano K,Kuboniwa M,Nakagawa I,et al.Comparison of inflammatory changes caused by Porphyromonas gingivalis with distinct fimA genotypes in a mouse abscess model.Oral Microbiol Immunol,2004,19(3):205-209. 被引量:1
  • 5Sugano N,Ikeda K,Oshikawa M,et al.Differential cytokine induction by two types of Porphyromonas gingivalis.Oral Microbiol Immunol,2004,19(2):121-123. 被引量:1
  • 6Nakagawa I,Amano A,Kuboniwa M,et al.Functional differences among FimA variants of Porphyromonas gingivalis and their effects on adhesion to and invasion of human epithelial cells.Infect Immun,2002,70(1):277-285. 被引量:1
  • 7Kato T,Kawai S,Nakano K,et al.Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype.Cell Microbiol,2007,9(3):753-765. 被引量:1
  • 8Umeda JE,Missailidis C,Longo PL,et al.Adhesion and invasion to epithelial cells by fimA genotypes of Porphyromonas gingivalis.Oral Microbiol Immunol,2006,21 (6):415-419. 被引量:1
  • 9Dorn BR,Burks JN,Seifert KN,et al.Invasion of endothelial and epithelial cells by strains of Porphyromonas gingivalis.FEMS Microbiol Lett,2000,187(2):139-144. 被引量:1
  • 10Watanabe K,Yamaji Y,Umemoto T.Correlation between cell-adherent activity and surface structure in Porphyromonas gingivalis.Oral Microbiol Immunol,1992,7(6):357-363. 被引量:1

同被引文献21

  • 1Hajishengallis G, Wang M, Liang S. Induction of distinct TLR2-mediated proinflammatory and proadhesive signaling path- ways in response to Porphyromonas gingivalis fimbriae [J]. J Im- munol, 2009,182 ( 11 ) :6690-6696. 被引量:1
  • 2Kraus D, Winter J, Jepsen S, et al. Interactions of adiponectin and lipopolysaccharide from Porphyromonas gingivalis on human oral epithelial cells [J]. PLoS 0ne,2012,7(2) :e30716. 被引量:1
  • 3Hajishengallis G. Porphyromonas gingivalis-host interactions: open war or intelligent guerilla tactics? [J]. Microbes Infect, 2009,11 (6-7) : 637-645. 被引量:1
  • 4Lamont R J, Chan A, Belton CM, et al. Porphyromonas gingivalis invasion of gingival epithelial cells [J]. Infect Immun, 1995,63 ( 1 O) : 3878-3885. 被引量:1
  • 5Brunner J, Scheres N, E1 Idrissi NB, et al. The capsule of Porphy- romonas gingivalis reduces the immune response of human gingi- val fibroblasts [J]. BMC Microbiol,2010,10:5. 被引量:1
  • 6Thompson H, Homer KA, Rao S, et al. An orthologue of Bacteroi- des fragilis NanH is the principal sialidase in Tannerella forsyth- ia [ J ]. J Baeteriol, 2009,191 ( 11 ) : 3623-3628. 被引量:1
  • 7Honma K, Mishima E, Sharma A. Role of Tannerella forsythia NanH sialidase in epithelial cell attachment [J]. Infect Immun, 2011,79( 1 ) :393-401. 被引量:1
  • 8Banerjee A, Van Sorge NM, Sheen TR, et al. Activation of brain endothelium by pneumococcal neuraminidase NanA promotesbacterial internalization [J]. Cell Microbiol, 2010, 12 (11) : 1576-1588. 被引量:1
  • 9Tong HH, Li D, Chen S, et al. Immunization with recombinant Streptococcus pneumoniae neuraminidase NanA protects chin- chillas against nasopharyngeal colonization [J]. Infect Immun, 2005,73 ( 11 ) : 7775-7778. 被引量:1
  • 10Tong HH, Blue LE, James MA, et al. Evaluation of the viru- lence of a Streptococcus pneumoniae neuraminidase-deficient mutant in nasopharyngeal colonization and development of oti- tis media in the chinchilla model [J]. Infect Immun, 2000, 68 (2) :921-924. 被引量:1

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