摘要
目的探讨不同颗粒大小的B-11CP植骨材料对腔隙性骨缺损骨修复的影响。方法将多孔β-TCP制备成粒径不同的三种形态：大颗粒组（A组），颗粒直径3．5-4．5mm，小颗粒组（B组），颗粒直径1．5～2．5mm，整块植骨组（C组），植骨块为圆柱体，直径6mm，高7mm，并以无填充组（D组）为空白对照，随机植入到60只新西兰大白兔胫骨近端圆柱状骨缺损（直径7mm，深6mm）处，于术后第2,4和8周取材。进行影像学、显微CT定量分析（micro—CT）、组织学检测分析，观察骨缺损区β-TCP降解和新骨形成情况，并进行统计学分析。结果影像学、显微CT及组织学检结果显示：在2周时，A组在新生骨生成量方面仅仅在2周时少于C组，多于其他两组；4周时A组和C组一致，多于其他两组；8周时A组为最多的一组。在降解速率上，三组植入物组2周、4周都趋于平缓，B组在8周时显示降解速率增快。结论多孔β—TCP的颗粒大小对兔长骨腔隙性骨缺损的修复产生影响，适当调整植骨粒径将有利于新骨的形成。
Objective To observe the effect of various sizes of ~ificial bone on conduction velocity of bone,explore the proper filling quantum and shorten the time of bone healing. Methods According to the different shape of β-tricalcium phosphate, the artificial bone was divided into four groups：group A： macro-bead filling with light mass, B： microaggregate filling with medium mass, single circular cylinder fining with heavy mass, D： blank group with no filling as control. These filling was transplanted into the round gap bone defect area with the diameter of 7 mm and deep of 6 mm in the medial surface of proximal tibia of 60 New Zealand rabbits, and then after the operation of 2,4,8 weeks the specimen was drawn and observed by the human eyes, X-ray and decalcification bony tissue section with triad celour staining. At last the quantum of new bone formation and material degradation was detected by micro CT.Results The quantity of new bone formation in group A was less than group C in 2 weeks but more than other 2 groups and equal to group A in 4 weeks, at last surpass the group C as the most group. As to the velocity of degredation, 3 transplantation groups were mild in 2,4 weeks, in the 8 weeks, group B was faster than others.Conclusion Macrobead filling with big mass evidently promotes the new bone formation in the rabbits live test and its degradation velocity is stable.
出处
《中国骨与关节损伤杂志》
2010年第7期602-605,共4页
Chinese Journal of Bone and Joint Injury
基金
陕西省自然科学基金资助项目[2006k12-G3(3)]
关键词
骨缺损
骨修复
Β-TCP
兔
Bone defect
Bone repair
β-tricalcium phosphate
Rabbit
