摘要
姜瘟病是由茄科雷尔氏菌（Ralstonia solanacearum）引起的一种细菌性病害,是世界生姜产区中普遍发生的一种毁灭性土传病害。研究表明,从发病田中未发病植株的根际土壤中分离得到的蜡状芽孢杆菌菌株L1（B.cere-us,L1）对茄科雷尔氏菌等8种病原菌具有较强的拮抗能力,具有广谱抗性。经Econo-Pac Q离子交换柱纯化、SDS-PAGE分析,从菌株L1得到一个表观分子量约为40 kDa的拮抗蛋白Lp,该蛋白具有光稳定性,在pH 6.0-8.0,温度20-60℃条件下均表现出对茄科雷尔氏菌的极强拮抗能力,但经胰蛋白酶处理后失活。实验表明,0.38 mg/mL为该蛋白的最低拮抗浓度。
Ginger wilt,caused by Ralstonia solanacearum,is one of the bacterial wilt diseases and a destructive disease in ginger production worldwide.In the present study,L1,an antagonistic bacterium belonging to Bacillus cereus,isolated from the ginger-growing soil,could strongly inhibit the growth of R.solanacearum ect.The antagonistic protein,Lp,produced by L1 was isolated and purified by Econo-Pac Q Ion-exchange chromatography column,and was subjected to the 12% SDS-PAGE and showed only a single band with about 40 kDa.Lp showed the strongest antagonistic activity to R.solanacearum under the conditions of pH 6.0～8.0 and 20～60 ℃.The results also revealed that Lp showed inactivation under light and after being digested by trypsin.
出处
《四川农业大学学报》
CSCD
北大核心
2010年第2期196-199,共4页
Journal of Sichuan Agricultural University
基金
四川省科技厅公益研究计划项目(2008FG0017)
四川省教育厅项目(07ZS001)
关键词
姜瘟病菌
芽孢杆菌
抗菌蛋白
性质
Ralstonia solanacearum
Bacillus cereus
antagonistic protein
character
