摘要
目的探讨血浆血小板α－颗粒膜蛋白（ＧＭＰ－１４０）及血管性假血友病因子（ｖＷＦ）在肝硬化患者血中变化的临床及病理生理意义。方法采用双抗体夹心固相酶免疫试验方法分别定量测定４０例肝硬化患者与３１例对照者血浆ＧＭＰ－１４０及ｖＷＦ水平。结果发现肝硬化患者血浆ＧＭＰ－１４０、ｖＷＦ水平比对照组有非常显著的增高（Ｐ＜００１），且发现肝硬化患者ＣｈｉｌｄＢ、Ｃ级均较ＣｈｉｌｄＡ级的ＧＭＰ－１４０水平显著升高（Ｐ＜００１），有上消化道出血组比无出血组的血浆ＧＭＰ－１４０、ｖＷＦ水平亦明显增高（Ｐ＜００５），同时还发现肝硬化组血浆ＧＭＰ－１４０、ｖＷＦ水平与血小板计数之间的关系均不相关（ｒ分别为－００３４９及－０１６２１，Ｐ＞００５）。结论肝硬化患者血浆ＧＭＰ－１４０、ｖＷＦ的升高可作为体内血小板活化与肝脏血管内皮细胞损伤的指标，可用来反映肝损伤状况及上消化道出血倾向。
Aims To explore the clinical and pathophysiologic significance of change of plasma granule membrane protein-140(GMP-140) and von willebrand factor(vWF) in patients with liver cirrhosis.Methods GMP-140 and vWF were detected in 40 patients with liver cirrhosis and 31 controls using enzyme-linked immunosorbent assay(ELISA).Results The level of GMP-140 and vWF were significantly increased in cirrhosis in comparison with controls(P<0 01).The level of GMP-140 was significantly increased in cirrhosis with child B and C in comparison with Child A(P<0.01).The level of GMP-140 and vWF were much higher in cirrhosis with upper gastrointestinal bleeding than without(P<0.05).Moreover,there was no significant correlation between the GMP-140 or vWF and the platelet count in cirrhosis(r=-0.0349 and -0.1621,respectively,P>0.05).Conclusions The elevation of GMP-140 and vWF in cirrhosis (1) can be a indicator of activation of platelet in vivo and injury in the hepatic vascular endothelium and (2) may reflect the degree of liver injury and tendency of upper gastrointestinal bleeding.
出处
《中国实用内科杂志》
CAS
CSCD
北大核心
1999年第1期34-36,共3页
Chinese Journal of Practical Internal Medicine
