摘要
目的分析虎杖苷吸入剂对支气管哮喘大鼠模型的干预效果。方法以10%的卵蛋白(OVA)和3%的氢氧化铝混合液腹腔注射实验大鼠2次致敏,然后以2%的OVA诱喘并进行虎杖苷和氨茶碱干预及生理盐水对照实验两周。观察实验大鼠诱喘和药物干预后的行为变化和肺组织切片、检测各组大鼠心室血中白细胞(WBC)、嗜酸性粒细胞(EOS)、嗜碱性粒细胞(BASO)、中性粒细胞(NEUT)和淋巴细胞(LYM)的数量变化。结果在诱喘过程中,虎杖苷干预组大鼠的哮喘症状得到明显改善,虎杖苷干预组大鼠EOS、LYM和MONO数量[(0.1339±0.01794)×109/L,(6.5440±0.49530)×109/L,(0.2148±0.08287)×109/L]较哮喘对照组大鼠的EOS、LYM和MONO[(0.1948±0.02454)×109/L,(8.0090±0.71166)×109/L,(0.2946±0.14257)×109/L]有所下降(P<0.05,P>0.05,P>0.05),且与正常对照组水平(0.1257±0.02454,6.9610±0.97243,0.1974±0.11765)接近。结论虎杖苷干预能够有效改善哮喘大鼠模型的哮喘症状。
ObjectiveTo detect the effects of the polydatin intervention in bronchial asthma rats. MethodsThe rats were injected with 1ml of the mix solution of 10% OVA and 3% aluminum hydroxide twice. And then, they were induced bronchial asthma with 2% OVA, and intervened with the polydatin solution and aminophyllin injection or controlled with saline for 2 weeks. The rat actions in inducing and intervening the asthma, and the rat lung session slides were observed. The numbers of WBC, EOS, BASO, NEUT and LYM in the ventricle blood were calculated. ResultsPolydatin intervention could obviously improve the asthmatic symptom. The number of EOS, LYM and MONO in the polydatin intervenient group（0.1339±0.017946, 5440±0.49530, 0.2148±0.08287） were lower than those in asthmatic group （0.1948±0.02454,8.0090±0.71166,0.2946±0.14257; P〈0.05, P〉0.05, P〉0.05）, and decreased to normal level in control goup（0.1257±0.02454, 6.9610±0.97243, 0.1974±0.11765）. ConclusionThe polydatin can significantly improve the asthmatic symptom of rat models.
出处
《医学研究杂志》
2010年第6期73-76,F0003,共5页
Journal of Medical Research
基金
广州市医药卫生科技项目(2008-YB-027)
广州市中医药
中西医结合科技项目(2009-A-05)
关键词
支气管哮喘
虎杖苷
炎性细胞
干预
Bronchial asthma Polydatin Inflammatory cell Intervention
