摘要
目的：应用ＲＰ－ＨＰＬＣ测定胃苏冲剂中柚皮甙含量。方法：用ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ柱，甲醇醋酸水（３５∶４∶６１）为流动相，检测波长２８３ｎｍ。结果：柚皮甙量在０．１２～１．０２μｇ范围内与色谱峰面积呈线性关系；回归方程＾Ｙ＝１７４１４０．３２Ｘ＋４３．３５，ｒ＝０．９９９７；平均加样回收率及ＲＳＤ分别为９８．７％和０．９８％。结论：该法建立了胃苏冲剂质量控制标准。
OBJECTIVE:To determine the content of naringin in Weisu Granule with RP-HPLC method.METHOD:The stationary phase was Shim pack CLC-ODS column;the mobile phase was methanol-aceticacid-water (35:4:61);and the test wavelength was 283nm.RESULT:The content of naringin had a linear relationship to the area of the chromatographic peak within the range of 0.12～1.02 μg.The regression equation was =174140.32X+43.35,r=0.9997;the average recovery rate and RSD were 98.7% and 0.98% respectively.CONCLUSION:This method can serve as the quality control standard for Weisu Granule.
出处
《南京中医药大学学报》
CAS
CSCD
1999年第2期93-94,共2页
Journal of Nanjing University of Traditional Chinese Medicine
