摘要
分离SD大鼠胰岛接种于24孔板中,用不同浓度的葡萄糖和白藜芦醇分别培养1 h或24 h,结果表明白藜芦醇孵育大鼠胰岛1 h可呈剂苗依赖地抑制大鼠高糖刺激的胰岛素分泌,1、10和100 μmol/L白藜芦醇可以分别使胰岛素的分泌降低10%、35%（P〈0.05）和80%（P〈0.01）.显微离子成像技术爪10μmol/L的白藜芦醇可以使高糖引起的β细胞内Ca^2＋浓度的升高减少60%（P〈0.05）.白藜芦醇可使软脂酸孵育24 h大鼠胰岛的胰岛素分泌恢复到对照组的75%（P〈0.01）,提示白藜芦醇短期可通过调控细胞内的Ca^2＋浓度,而抑制原代胰岛高精刺激的胰岛素分泌,长期可改善软脂酸引起的β细胞损伤.
Isolated freshly rat islets were transferred to 24-well plates and incubated with different concentrations of glucose or resveratrol for 1 or 24 h.The results showed that resveratrol dose-dependently inhibited glucose-stimulated insulin secretion from isolated rat islets after 1 h incubation,with 10%,35%,and 80% （P〈0.05 or P〈0.01） decrease at the concentrations of 1,I0,and 100 μmol/L.10 μmol/L resveratrol decreased the intracellular calcium concentration by 60% （P〈0.05）.After incubation for 24 h,resveratrol increased palmitatesuppressed insulin secretion to 75% （P〈0.01） of control.These results suggest that resveratrol acutely inhibits insulin secretion from primary pancreatic islet via regulating intracellular calcium ion concentration,and in the long run resveratrol may protect β-cells from lipotoxicity.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2010年第6期495-497,共3页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金（307000382,30771021）
关键词
白藜芦醇
胰岛素分泌
钙离子
脂毒性
Resveratrol
Insulin secretion
Calcium ion
Lipotoxicity
