摘要
采用海藻酸铝固定化糖化酶,对固定化酶和游离酶的特性进行了比较。结果表明,固定化酶米氏常数Km为13.72 mg/mL,最适温度为65℃,半衰期为100.7 d,固定化糖化酶的活力回收率达61.8%。
The method of immobilized glucoamylase by alginate aluminium was used.The characteristics of immobilized glucoamylase compared with that of free glucoamylase.The results showed that the immobilized glucoamylase＇s Michaelis constant was 13.72 mg/mL,the optimum temperature was 65 ℃,the half life was 100.7 d and the activity recovery rate was 61.8%.
出处
《应用化工》
CAS
CSCD
2010年第6期886-888,共3页
Applied Chemical Industry
关键词
海藻酸铝
固定化
糖化酶
特性
alginate aluminium
immobilization
glucoamylase
characteristic
