摘要
在摇瓶中用液体培养基培养长春花（Catharanthusroseus（L．）G．Don）激素自养型细胞系C20hi，比较了不同初始糖浓度、接种量、初始pH值、光照时间、光质和摇床转速对该培养细胞生长、阿玛碱积累和释放的影响。结果表明，此细胞系具有较强的环境耐受性；一定范围内初始糖浓度增加，有利于细胞生长和生物碱生成；最适的接种量是60gFW／L；一定范围内改变培养基pH值，对生物碱生成没有显著影响；照光后生物碱生成量下降，红光较蓝光更有利于生物碱生成；最适的摇床转速是120r／min。
This paper deals with nutritional and environmental factors on cell growth and alkaloid accumulationand release of full habituated Catharanthus roseus (L.) G. Don cell line C20hi in suspension cultures. The effects of different initial sucrose concentration, amount of inocula, initial pH values, illuminating time, wavelength of light on cell growth and ajmalicine production and release of the cell suspensions were compared.C20hi is a cell line relatively stable to environmental changes. High concentration of initial sucrose increasedthe biomass accumulation and alkaloid production. The suitable inoculum of C20hi was 60g FW/L. Increasingthe initial PH value in the range of 4-7 had no obvious effect to alkaloid production. light had a detrimentaleffect on alkaloid production. The cells under ho light could accumulate more alkaloids than that under bluelight. The shaker speed of 120 r/min was found to be most suitable for C20hi suspensions.
基金
国家自然科学基金
国家中医药管理局资助
上海市教育委员会资助
关键词
激素自养型细胞
营养和环境因子
长春花
细胞培养
Full habituated cells, Nutritional and environmental factors, Catharanthus roseus, Cell culture
