摘要
运用化学诱变法从产甘油假丝酵母获得１９株渗透压突变株。对其生理及发酵性能的研究结果显示，所有渗透压敏感突变株的甘油产率皆显著降低并巨大多数渗透压敏感突变株的糖利用速度减慢．耐渗透压性能提高突变株的糖利用率几乎与亲株相同而甘油发酵性能改变呈现多样性，突变株如ＷＬ－ＯｓｍＤ、ＷＬ－ＯｓｍＦ以及ＷＬ-ＯｓｍＨ甘油转化率极度降低而突变株ＷＬ－ＯｓｍＢ的甘油耗糖转化率接近亲株。
?Osmotolerance is one of the most important physiological characteristics that Candida glycerolgenesis has. To make clear what role the osmotolerance plays on the over-synthesis of glycerol, we screened out 10strain of osmosensitive mutants and 9 strain of more osmotolerant mutants from Candida glycarolgenesis bychemical mutagenesis. Cultured the osmotic mutants in the broth containing different concentration of glucose,glycerol or sedium chloride, osmosensitive mutant WL-Osm5 showed more sensitive to the decrease of wateractivity but osmotolerant mutant WL-OsmB did the same as the parent. All the osmosensitive mutants decreasedtheir glycerol productivity greatly but the change of glycerol productivity of osmotolerant mutants varied fromnear the same glycerol yield as the parent the mutant WL-OsmB showed to remarkable decrease the mutantsWL-OsmD, WL-OsmF and WL-OsmH showed.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第1期24-26,共3页
Microbiology China
基金
国家"九五"攻关项目
关键词
产甘油假丝酵母
发酵
甘油
耐高渗压
Candida glycerolgenesis , Osmotic mutant, Fermentation, Glycerol, Osmotolerance
