摘要
采用增强UV-B辐射矮牵牛,研究增强UV-B辐射下矮牵牛发生变异的生理生化机制.结果表明,变异矮牵牛丙二醛(Malondialdehyde,MDA)含量显著低于对照矮牵牛(P<0.01),而黄酮类化合物含量显著高于对照矮牵牛(P<0.01),高效液相色谱图的最高主峰保留时间分别为11.818min(对照矮牵牛)和13.783min(变异矮牵牛).同时应用随机扩增多态性DNA(Random amplifi ed polymorphic DNA,RAPD)技术对矮牵牛进行检测分析,发现引物在对照矮牵牛及变异矮牵牛之间扩增出差异性多态条带,表明增强UV-B辐照引起矮牵牛基因组DNA发生变异.
The physiological and RAPD variations and phenotype change of Petunia hybrida caused by exposure to enhanced UV-B radiation with its seedlings were studied.The results showed that the MDA content in the variant plants of P.hybrida was markedly lower than that in the control plants,while the flavonoids content of the variant plants was markedly higher than that of the control.The retention time of HPLC high peak was 11.818 and 13.783 min for the control and variant plants,respectively.RAPD（Random amplified polymorphic DNA） variation of the control and variant plants induced by the enhanced UV-B radiation suggested that random primers would have been amplified with different DNA polymorphism bands.Consequently,the enhanced UV-B radiation could cause mutation of genome DNA in P.hybrida.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2010年第3期323-327,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(No.30670366)
河南省生物化学与分子生物学重点学科和河南师范大学青年科学基金项目(No.2008qk15)资助~~
