摘要
【目的】探究脂肪酸合成酶(FAS)基因3′端非翻译区(3′-UTR)在绵羊地方品种中的遗传多态性,为进一步揭示地方绵羊品种间遗传分化、开展肉质性状的关联分析和表达调控等研究提供依据。【方法】采用PCR-SSCP和DNA测序技术对兰州大尾羊(38只)、滩羊(58只)、甘加羊(40只)、欧拉羊(30只)和乔科羊(39只)五个地方绵羊品种205只个体的脂肪酸合成酶(FAS)基因3′-UTR进行单核苷酸多态性(SNPs)检测和遗传多态性分析。【结果】在这五个地方绵羊品种的FAS基因3′-UTR区中,有951位点和1005位点2个多态位点;两位点分别存在AA、Aa、aa和EE、Ee、ee各三种基因型,而aa和ee基因型未检测到;AA和EE基因型频率高于Aa和Ee基因型频率,基因型AA和EE为优势基因型;A和E两个等位基因频率高于a和e等位基因频率,为优势等位基因。适合性检验表明,这5个地方绵羊品种在951位点和1005位点均处于Hardy-Weinberg平衡状态。独立性检验表明:在951位点,兰州大尾羊与滩羊、欧拉羊、甘加羊和乔科羊之间表现为差异显著(0.01<P<0.05);滩羊与欧拉羊、甘加羊和乔科羊之间表现为差异极显著(P<0.01);欧拉羊、甘加羊和乔科羊,甘加羊与乔科羊均表现为差异不显著(P>0.05);在1005位点兰州大尾羊、滩羊、欧拉羊、甘加羊和乔科羊品种之间均表现为差异不显著(P>0.05)。测序结果表明,951位点在FAS基因在其转录产物mRNA的951位所对应处有一处C→T突变;1005位点在FAS基因在其转录产物mRNA的1005位所对应处有一处A→G突变。FAS基因mRNA二级结构预测结果显示:951位点的突变能导致其二级结构发生了明显的改变,而1005突变不会改变其二级结构。【结论】五个地方绵羊品种在FAS基因3′-UTR区有951位点(C→T)和1005位点(A→G)两个SNPs,2位点均处于Hardy-Weinberg平衡状态;基因型频率分布的独立性检验结果表明,在951位点,兰州大尾羊与滩羊、欧拉�
【Objective】 Genetic polymorphisms of FAS gene in sheep were detected in order to provide a theoretical foundation for further studying on the genetic differentiation among sheep breeds,association analysis on sheep meat quality traits and polymorphism,location and expression regulation of sheep FAS gene,and so on. 【Method]】 PCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms （SNPs） and analyze the genetic polymorphisms at 3＇-UTR of FAS （Fatty acid synthase） gene of five Chinese sheep breeds including Lanzhou fat-tailed sheep （LT,38）,Tan sheep （Tan,58）,Oula sheep （OL,30）,Ganjia sheep （GJ,40） and Qiaoke sheep （QK,39） in this study. 【Result】 2 SNPs （951SNPs and 1 005SNPs） were observed in 3＇-UTR of sheep FAS gene,and 3 genotypes （AA,Aa,aa and EE,Ee,ee） were detected,respectively. AA,Aa and EE,Ee were observed in all the 5 Chinese sheep breeds,but aa and ee genotypes were not detected in any breed. AA and EE were the genotype with the highest frequency in all of the sheep breed. The predominant alleles were alleles A and E,whose frequency was higher than a and e in all the 5 breeds. Compatibility test indicated that Lanzhou fat-tailed sheep,Tan sheep,Oula sheep,Ganjia sheep and Qiaoke sheep were all in the Hardy-Weinberg equilibrium at 2 SNPs. Independence test indicated that：at 951 SNPs site the genotype distribution was obviously different between Lanzhou fat-tailed sheep and other all sheep breeds （Tan sheep,Oula sheep,Ganjia sheep and Qiaoke sheep） （0.01P0.05）,and it was extremely obviously different between Tan sheep and other sheep breeds （Oula sheep,Ganjia sheep and Qiaoke sheep）（P0.01）,while it was not obvious between Oula sheep and other two sheep breeds （Ganjia sheep and Qiaoke sheep）（P0.05）. At 1 005 SNPs site it was not obvious between all of the 5 sheep breed （P 0.05）. Sequencing analysis showed that 2 mutations （GSA） occurred at the 951 site （C→T） and 1 005 site （A�
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第13期2784-2792,共9页
Scientia Agricultura Sinica
基金
兰州市科技计划项目(2006-2-59)
甘肃省农业生物技术研究与应用开发项目(GNSW-2009-13)
国家民委科研项目(2009-158-09XB02)
