摘要
采用微波协同酶法提取玉米须多糖并对其工艺进行优化。通过单因素试验和正交试验,确定微波协同酶法提取的最佳工艺条件:微波功率500W、微波处理时间2min、液料比30:1(mL/g)、纤维素酶用量1.5%、酶解温度50℃、酶解时间40min、pH5.0,在此工艺条件下,多糖提取率为8.12%。
The present study aimed at developing an optimal procedure for the extraction of polysaccharides from corn silk hydrolyzed with a commercial cellulase preparation under microwave assistance. The optimal values of crucial technological parameters for improved polysaccharides yield were determined by one-factor-at-a-time and orthogonal array design methods as follows： microwave powder 500 W for 2 min treatment, water/material ratio 30：1 （mL/g）, enzyme dosage 1.5%, pH 5.0 and hydrolysis temperature 50 ℃ for a hydrolysis duration of 40 min. Under such conditions, a maximum polysaccharides yield of up to 8.12% was obtained.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第10期42-46,共5页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD27B07)
关键词
玉米须
多糖
提取
微波
纤维素酶
corn silk
polysaccharides
extraction
microwave
cellulase
