摘要
用聚合酶链反应 ,从水鹿、坡鹿、梅花鹿和马鹿等四种鹿属动物中分别扩增出线粒体DNA的细胞色素 b基因片段 ,并测定得到 367bp的碱基序列 ,它们之间的序列差异在 4 .0 9%～ 7.0 8%之间。用 NJ法、最大简约法和最大似然法进行分子系统进化树的构建和分析并得出 ,水鹿与坡鹿、梅花鹿和马鹿约在 2 4 0～ 2 80万年前分化的 ,梅花鹿和马鹿大约在 1 60万年前左右分化。
Hidetoshi TAMATE (Department of Biotechnology,Senshu University of Ishinomaki Japan) Ryuichi MASUDA Jyunko NAGATA Noriyuki OTAISHI (Faculty of Science and Graduate School of Veterinary Medicine, Hokkaido University Japan) China is one of the major distribution and evolution centers of deer. The phylogenetic study of deer in China will further contribute to understanding of the deer's origin and evolution. The phylogenetic relationships among four species of deer in China were studied with molecular biological technique,in this study. The 367bp mtDNAs cytochrome b were sequenced using PCR from Sambar(Cervus unicolor),Eld's deer(C.eldi),Sika deer(C.nippon) and Red deer(C.elaphus). The sequence divergence of cytochrome b was 4.09%～7.08%. The phylogenetic tree was constructed and analyzed by NJ method,Maximum parsimony and Maximum likelihood. The results indicated that the four species of deer constructed a monophyletic group. Sambar diverged with Eld's deer,Sika deer and Red deer before 2.4 ～2.8 Mya,and Sika deer and Red deer diverged each other before 1.6 Mya.
出处
《动物学报》
SCIE
CAS
CSCD
1999年第1期99-105,共7页
ACTA ZOOLOGICA SINICA
