摘要
目的:了解新型肝炎病毒—TT病毒在中国的流行情况,探讨其核酸变异性。方法:收集19例健康体检发现谷丙转氨酶升高者的血清标本,采用PCR方法检测TT病毒的DNA。所得PCR产物再采用限制性片段长度多态性分析和DNA序列分析方法进行验证。最后将测序结果与GenBank中的一些分离株进行比较,分析它们的同源性。结果:19例转氨酶升高者中,2例PCR检测呈阳性反应,其产物经限制性片段长度多态性分析证实为特异性产物。DNA序列分析显示,2个阳性产物间的核酸同源性为987%,与Takahashi等分离株和Okamoto等分离株相比,同源性分别为987%和892%。两个TTVDNA片段中均存在精氨酸取代丝氨酸变异。结论:在中国广东人群中存在TT病毒感染,其核酸序列与Takahashi等在日本得到的分离株高度同源。因而,设计PCR引物时宜选择Takahashi等分离株为标准。精氨酸取代丝氨酸变异的意义需进一步研究。
Objective: To investigate the incidence of a new hepatitis virus (TT virus) infection in Guangdong, China, and analyze its nucleic acid variance. Methods: PCR was used in the detection of TTV DNA in 19 serum samples from elevated alanine aminotransferase persons in their routien medical check up. PCR products were confirmed by RFLP and DNA sequencing. Nucleotide sequence homology was analyzed by comparison with major isolates from GenBank. Results: TTV DNA was detected in 2 out of 19 cases by PCR. PCR products were verified to be specific. DNA sequencing showed that the homology rate of these 2 fragments was up to 98.7%. When compared with Takahashi's isolates and Okamoto's isolate, the homology rates were 98.7% and 89.2% respectively. A unique amino acid replacement of serine by arginine was found in both fragments. Conclusions: There were TTV infections in Chinese population . In China these viruses share high homology with Takahaski’s strains which might be good standards for primer designation. The significance of the unique replacement of amino acid remained to be studied.
出处
《中山医科大学学报》
CSCD
1999年第1期65-68,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省自然科学基金
关键词
病毒性肝炎
病因学
TT病毒
谷丙转氨酶
hepatitis, viral, human/etiology
alanine aminotransferase/blood
TT virus