摘要
对鳝鱼皮中胶原蛋白的最佳提取工艺条件进行了研究。以超声提取时间、胰蛋白酶加入量、酶解时间、提取温度、pH5个因素对提取工艺条件进行了优化,结果表明,最佳提取工艺条件为:酶加入量125U/g、pH=7.2、温度35℃、超声波辅助提取时间3min、酶解时间7h,胶原蛋白的提取率达92.4%。利用紫外光谱和傅立叶变换红外光谱对胶原蛋白进行分析,结果表明,胶原蛋白的紫外特征吸收波长为226nm,结构中含有肽键,羰基,羧基等蛋白质的特征官能团。
The extraction conditions of the collagen in the skin of Monopterus albus are investigated in this paper.The extraction conditions are optimized by the experiment to five factors of supersonic extraction time,the addition of trypsin,temperature,pH value and the decomposition time of trypsin.The result shows that the optimum conditions are the supersonic extraction time 3min,the amount of trypsin 125 U/g,pH=7.2,temperature 35 ℃,the decomposition time of enzyme 7 hours,and the extraction rate of collagen is 92.4%.Collagen has been analyzed by UV and IR.The result shows that the λmax of product is 226 nm and the functional groups of collagen have peptide bond,carbonyl and carboxyl etc.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2010年第6期553-557,561,共6页
Fine Chemicals
关键词
鳝鱼皮
胶原蛋白
胰蛋白酶
分离提纯技术
the skin of monopterus albus
collagen
trypsin
separation and purification technology
