摘要
研究了ＦＣＳ，１７β－Ｅ２，ＦＳＨ，ＬＨ对山羊卵泡卵母细胞成熟的作用，及培养时间对卵母细胞核和细胞质成熟的影响。结果表明，添加体积分数为１０％～１５％的ＦＣＳ，１～２ｍｇ·Ｌ－１１７β－Ｅ２，２０～５０ｍｇ·Ｌ－１ＬＨ和１０ｍｇ·Ｌ－１ＦＳＨ有利于提高卵母细胞核成熟，并放出第一极体。而成熟培养２０，２４ｈ的卵母细胞成熟率显著高于培养１６ｈ（Ｐ＜０．０５），核成熟后培养３～４ｈ有利于卵母细胞质成熟，对其后的受精、卵裂及胚胎的进一步发育有重大影响。且ＴＣＭ１９９＋质量分数为１０％～１５％的ＦＣＳ＋１～２ｍｇ·Ｌ－１１７β－Ｅ２＋１０ｍｇ·Ｌ－１ＦＳＨ＋２０～５０ｍｇ·Ｌ－１ＬＨ是卵母细胞成熟的理想培养系统。
The effects of FCS,culture time,17β estradial,FSH and LH on goat oocytes in vitro maturation were studied.The results suggested that an addition of 10%-15% FCS,1-2 mg·L -1 17β E 2,20-50 mg·L -1 LH and 10 mg·L -1 FSH was effective to improve oocyte nuclear maturation,and to extrude the first polar body.The maturation rate of oocytes cultured for 20,24 h was significantly higher than that of 16 h( P <0.05).After nuclear maturation,culture for 3-4 h was beneficial to oocyte cytoplasm maturation,and had a significant effect on fertilization,cleavage and embryo development.An addition of 10%-15% FCS,10 mg·L -1 FSH 10-50 mg·L -1 LH and 1-2 mg·L -1 17β E 2 in the TCM199 medium was an ideal culture system for goat oocyte in vitro maturation.
出处
《西北农业大学学报》
CSCD
北大核心
1999年第1期14-18,共5页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
农业部生物技术重点项目
