摘要
采用16SrDNA文库和变性梯度凝胶电泳（DGGE）技术，对黄海西北部3个近岸站位沉积物中细菌群落多样性及空间分布特征进行了调查和解析。对表层沉积物16SrDNA序列统计表明，各站位细菌群落多样性很高，γ-和δ-变形菌纲分别占克隆序列总数的20％～32％，是沉积物中的绝对优势类群。DGGE图谱分析表明，同一站位中不同深度的细菌群落结构相似性较高，而不同站位间群落结构相差较远。研究表明在黄海西北近岸沉积物中细菌群落多样性较高，优势类群明显，在较小尺度范围内群落结构的垂直变化不明显。
16S rDNA clone library combining with denaturing gradient gel electrophoresis （DGGE） technique was used to reveal the bacterial community diversity and the spatial distribution characteristics from the three stations of the sediment of the northwest Huanghai Sea. The bacterial 16S rDNA libraries of the surface sediments showed that at all three stations composed high diversity of bacterial communities, 7-and 6-proteobacteria were the donminant groups, respectively, accounting for 20% and 32% of the total sequences. DGGE profiles showed that the bacterial community structure presented a high degree of similarity at the same stations at different depths, but demonstrated far distance from different stations. The present study shows that the bacteria from the coastal sediments have a high community diversity and possess dominant groups in bacterial community, but on a smaller scale, the vertical changes of community structure are not obvious.
出处
《海洋学报》
CAS
CSCD
北大核心
2010年第2期118-127,共10页
基金
水体污染控制与治理科技重大专项(2008ZX07208-009)
国家自然科学基金(40801193)
关键词
16S
rDNA文库
变性梯度凝胶电泳
细菌群落
16S rDNA clone library
denaturing gradient gel electrophoresis （DGGE）
bacterial community
