摘要
目的利用三维重建工具软件对小鼠大脑、海马结构进行三维重建,并对重建的图像进行观察和测量。方法获取小鼠大脑连续冷冻Nissl染色切片,进行图像预处理后,构建三维重建数据集。利用PhotoshopCS3软件对小鼠大脑冠状切片海马结构的不同区域填充不同的颜色。然后,利用3D-DOCTOR 4.0软件分别对上述连续切片图像进行配准、分割和三维重建,并对重建的脑及海马结构进行观察。结果利用小鼠脑连续冷冻Nissl染色切片图像可以对小鼠海马结构进行三维重建,并能立体观察海马下托、CA1、CA2、CA3、CA4区和齿状回等结构。结论利用小鼠脑的连续冷冻Nissl染色切片可以对小鼠脑及海马结构进行三维重建。
[ Abstract] Objective To make three-dimensional （3D） reconstruction of mouse brain and inner hippcampal formation （HF） with 3D software, and to observe and measure the reconstructed structures. Methods The Nissl stained mouse brain serial cryosections were obtained and pre-processed to construct the image dataset for 3D reconstruction. Using PhotoshopCS3 software, the different anatomic structures of HF in mouse coronary brain sections were fulfilled with different colors according to anatomy knowledge. The 3D-DOCTOR 4.0 software was used to make registration, segmentation and 3D reconstruction of mouse brain and HF, and to observe the reconstructed structures in personal computer （PC）. Results The serial Nissl stained cryosections could be used to make 3D reconstruction of mouse HF, and the subiculum, CA1, CA2, CA3, CA4, dentate gyrus （DG） , etc, could be observed. Conclusion The serial Nissl stained mouse cryosections could be used to make 3D reconstruction of mouse brain and HF.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第3期458-461,共4页
Acta Anatomica Sinica
基金
江苏省研究生创新培养工程资助项目(CX07s_035z)
南通大学医学院科研项目(yxy-200906)
关键词
海马结构
连续切片
3D-DOCTOR
三维重建
小鼠
Hippocampal formation
Serial section
3 D-DOCTOR
Three-dimensional reconstruction
Mouse
