摘要
目的观察缺血后处理对大鼠移植肝缺血再灌注损伤的保护作用并探讨其可能机制。方法建立SD大鼠原位肝移植模型,48只健康雄性SD大鼠随机分为再灌注损伤组和后处理组。再灌注损伤组供肝植入前后无特殊处理,后处理组供肝植入后门静脉完全再通前给予多次短暂复灌复停作为后处理。两组受体一半(n=6)于再灌注后2h处死,检测血清肝功能指标、肿瘤坏死因子α(TNFα)和中性粒细胞弹性蛋白酶(NE)水平以及肝组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量的变化;另一半(n=6)于再灌注后6h处死,留取肝组织制备HE染色切片,光镜下观察组织学变化。结果后处理组血清肝功能指标、炎性细胞因子水平及肝组织过氧化物含量均显著低于再灌注损伤组,而肝组织抗氧化酶活力显著高于再灌注损伤组,肝组织的病理改变也明显轻于再灌注损伤组。结论缺血后处理通过有控制地复氧,减少氧自由基的产生,抑制效应器TNFα、NE的合成及释放,发挥对大鼠移植肝缺血再灌注损伤的保护作用。
Objective To study the protective efficacy of ischemic post-conditioning on ischemic reperfusion injuries of rat liver graft and determine the possible mechanism.Methods The model of orthotopic liver transplantation was established in Sprague Dawley （SD） rats. 48 healthy male rats were randomly assigned into ischemic reperfusion injury group and ischemic post-conditioning group. There was no special care given to ischemic reperfusion injury group before or after donor liver was implanted,however several brief ischemic reperfusions were given for ischemic post-conditioning group before portal vein was completely re-canalized. Half the recipients of the two groups （n=6） were sacrificed in two hours after reperfusion. Liver function tests,tumor necrosis factor-α （TNF α） and neutrophil elastase （NE） in serum and the contents of superoxide dismutase （SOD） and malondialdehyde （MDA） in hepatic tissue were measured. Other six recipients were sacrificed in six hours after reperfusion and hepatic tissue sections were observed with light microscope. Results Liver function tests,cytokines and peroxide product contents of ischemic post-conditioning group were much smaller than that of ischemic reperfusion injury group. The antioxidase content in hepatic tissue of ischemic post-conditioning group was significantly higher than that of ischemic reperfusion injury group. The pathological change of ischemic post-conditioning group was slighter and significantly different from the ischemic reperfusion injury group.Conclusion Ischemic post-conditioning reduces the ischemic reperfusion injury of rat liver graft by decreasing the production of oxygen free radical and restraining the synthesis and release of TNF α and NE.
出处
《临床肝胆病杂志》
CAS
2010年第3期317-318,322,共3页
Journal of Clinical Hepatology
基金
国家自然科学基金项目(No.30700810)
关键词
肝移植
再灌注损伤
肿瘤坏死因子α
白细胞弹性蛋白酶
liver transplantation
reperfusion injury
tumor necrosis factor-alpha
leukocyte elastase
