摘要
目的评价肺内炎症免疫效应细胞释放的细胞因子对肺结节病发病机理的影响。方法利用微孔滤膜和酶联免疫法，检测１１例肺结节病和７例特发性肺纤维化（ＩＰＦ）患者血清和支气管肺泡灌洗液（ＢＡＬＦ）中肿瘤坏死因子（ＴＮＦα）和中性粒细胞趋化因子（ＮＣＦ）水平，并与８名健康非吸烟者对照。结果肺结节病和ＩＰＦ患者ＢＡＬＦ中的ＴＮＦα为１１．９±３．２、１１．７±３．０ｎｇ·Ｌ１，ＮＣＦ水平以每１０个高倍视野细胞数表示，为１９１±５１、２０３±４４，明显高于正常对照组的６．５±１．４ｎｇ·Ｌ１和８２．５±４５．４（Ｐ＜０．０１）。肺结节病患者ＢＡＬＦ中ＴＮＦα与淋巴细胞、ＩＰＦ患者ＮＣＦ与中性粒细胞呈明显正相关（ｒ＝０．７３和ｒ＝０．８９Ｐ＜０．０１）。结论ＴＮＦα和ＮＣＦ在肺结节病和ＩＰＦ发病机理中起着重要作用，并可作为判定疾病活动性的一项重要参考指标。
Objective To evaluate the role of cytokines released by the inflammatory cells and immunocytes from patients in pathogenesis of developing sarcoidosis. Methods With well microchemotaxis and allergy immunology, the level of tumor necrosis factor α (TNF α) and neutrophil chemotactic factor (NCF) was measured in the serum and BALF of 11 sarcoidosis and 7 IPF and 8 normal subjects (non smokers). Results The levels of TNFα (11.9± 3.2、11.7±3.0 ng·L 1 ) and NCF (191±51、203±44 cell·10HP 1 )of BALF in the patients with sarcoidosis and IPF were significantly higher than these in control group ( P <0.01)and were higher than those in serum. The level of TNF α in the BALF of patients with sarcoidosis was positively correlated with the percentage of lymphocytes ( r =0.73, P <0.01). The activity of NCF in the BALF of patients with IPF was positively correlated with the percentage of neutrophils ( r =0.89, P <0.01). Conclusions It indicated that TNF α and NCF might play an important role in the pathogenetic process of the sarcoidosis and IPF, and can act as the marker of activity of these diseases.
出处
《中华结核和呼吸杂志》
CSCD
北大核心
1999年第1期37-39,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
结节病
BALF
肿瘤坏死因子
NCF
肺结节病
Sarcoidosis
Bronchoalveolar lavage fluid
Tumor necrosis
Neutrophil chemotactic factor
