摘要
目的研究乌头类生物碱对大鼠视网膜神经细胞细胞增殖周期和bcl-2基因表达的影响,探讨乌头类中药神经毒性的作用机制。方法体外培养大鼠视网膜神经细胞,分别以0.5%的乌头碱、新乌头碱和次乌头碱作用细胞5min后,流式细胞仪检测。结果与阴性对照组比较,乌头碱作用组G2/M期细胞比例从2.3%增加到19.5%,细胞增殖活动被抑制;而bcl-2基因表达量显著增加,表达荧光均值从0.78增加到1.45。新乌头碱和次乌头碱作用组结果与乌头碱类似。结论在本试验条件下,bcl-2基因可能在乌头类生物碱的神经毒性作用机制中具有重要意义。
Objective To study the effect of aconitic alkaloid on cell cycle and bcl-2 gene expression of rat retina neuron,and explore the mechanism of aconitic neurotoxicity. Methods Rat retina neuron was cultivated in vitro,and treated with aconitine,mesaconitine and hypaconitine for 5 minutes respectively,then determined with Flow Cytometer. Results Compared with negative control,the proportion of G2 /M cell in the group treated with aconitine increased from 2. 3% to 19. 5% , which meant that cell proliferation had been inhibited significantly. In addition,the expression amount of bcl-2 protein in the group treated with aconitine significantly increased from 0. 78 to 1. 45. Similar results were also observed in the groups treated with meaconitine and hypaconitine. Conclusion Bcl-2 gene should play an important role in the mechanism of aconitic neurotoxicity.
出处
《毒理学杂志》
CAS
CSCD
北大核心
2010年第2期94-96,共3页
Journal of Toxicology
基金
国家重点基础研究发展计划(2006CB504705)
国家自然科学基金重点项目(30230410)
