摘要
以抗碱野生大豆Gs0001(♀)和碱敏感栽培大豆吉科豆1号(♂)的F2群体作为基因定位群体,通过BSA法(Bulked-segegant analysis,群分法),对大豆耐碱基因进行SSR分子标记定位。在分布于大豆20个连锁群的488对SSR引物中,筛选出7对与耐碱基因紧密连锁的标记,这7对标记位于G连锁群相近的区域。利用Mapmaker/EXP3.0分析,在最小似函数值LOD=14.0时,将耐碱基因定位于Satt298与Satt269之间,距离两个标记的遗传距离分别为1.3 cm和6.9 cm。此耐碱基因的贡献率是40.31%。
Using F2 of alkali-tolerant wild soybean Gs0001（♀） and alkaline-sensitive cultivated soybean‘＇Jikedou 1’（♂） as gene targeting groups,SSR Molecular Markers of soybean alkali-resistant genes were carried out by the BSA（Bulked-segegant analysis） method.In 488 pairs of SSR primers distributed in 20 linkage groups,7 pairs of markers closely linked with the alkali-resistant genes were screened out,which was near to G linkage group regions.Using Mapmaker/EXP3.0 analysis,the minimum function value LOD = 14.0,alkali-resistant gene was between Satt298 and Satt269.The genetic distances between the two markers were 1.3 cM and 6.9 cM,respectively.The alkali-resistant gene＇s contribution rate was 40.31%.
出处
《吉林农业科学》
CSCD
2010年第3期15-17,56,共4页
Journal of Jilin Agricultural Sciences
基金
国家重点基础研究发展计划(973计划前期)资助项目(2007CB116205
2004CB117203-3)
农业部生物资源保护与利用项目资助
