摘要
目的 比较自制可脱落球囊和自体血凝块行大面积肺动脉栓塞动物模型的异同.方法 健康绵羊18例,随机分为3组：血栓栓塞组（简称血栓组）、球囊栓塞组（简称球囊组）和空白对照组.所有动物均经全麻下监测有创肺动脉压、中心静脉压及外周动脉压.使用12 F薄壁指引导管置于右肺动脉,将自制可脱落球囊或自体血凝块经导管引入,建立肺栓塞动物模型.分别于栓塞术前、肺栓塞模型建立后0.5 h、1 h、2 h、4 h、6 h及8 h监测平均动脉压（MAP）、平均肺动脉压（MPAP）、心率、呼吸频率及外周血氧饱和度（SaO2）,动脉血氧分压（PaO2）和动脉血二氧化碳分压（PaCO2）.结果 2组建立大面积肺栓塞模型的绵羊均在注入自体血栓或球囊阻塞后经肺动脉造影证实为右侧肺栓塞.实验动物均存活8 h以上.动物模型建立后0.5 h～2 h出现心率、呼吸频率加快,SaO2及PaO2下降,肺动脉压增高,与空白对照组比较差异有统计学意义,但2种栓塞方法间对照差异无统计学意义.2 h后逐渐稳定,球囊组呼吸指标缓慢回升,血栓组则稳定于较低水平,球囊组与血栓组对照有统计学差异.结论 球囊栓塞较好的模拟肺栓塞的机械作用,血凝块较好的模拟血栓中活性物质的影响,可根据实验目的的不同而选择不同的模型复制方式.
Objective To contrast the massive pulmonary embolism of the animal model had been stabled by inserting the animal＇s own blood clots or through use of detachable latex balloons. Methods Eighteen healthy sheep were separated as three groups with random. We established the animal lung, PE model by inserting the animal＇ s own blood clots at right lung artery in 6 sheep, and 6 sheep were through used of detachable latex balloons to substitute the blood clots. Six healthy sheep were used as controls. Eachsheep was anesthetized. Both sides of the region inguinalis were applied 21 transfixion pins to one femoralartery and two femoral veins on the left side, and one femoral vein on the right side. All vessels were inducted into the 5 F vagina vasorum except one vein for which 12 F was inducted 50 ml blood was taken from vagina vasorum from each animal and stabilized for 2 h for later use as a body blood clot. Guided and visualized by X-ray, a 5 F pigtail catheter was inserted on the left side to measure arterial pressure, central venous pressure and pulmonary arterial pressure; a 12 F guiding catheter was then inserted through right side femoral vein into the right lung artery to inject 8 ml 350 mg I ultravist at 6 ml/s for pulmonary arteriography,After the animal was stabilized for 30 min, we input their own body blood clots （0.6 ml/kg） using a 12 F guiding catheter or, alternatively, to release a self-made, detachable latex balloon. We measured arterial pressure, central venous pressure, pulmonary arterial pressure, heart rate, blood oxygen saturation, partial pressure of oxygen in artery and partial pressure of carbon dioxide in artery etc. Of the animals both immediately before inputting their own body blood clots or detachable latex balloons, immediately afterwards,immediately before interfering with airflow and at 3 min,6 min,10 min,15 min,20 min,30 min, 45 min and 60 min after the interference. Results Heart and breath rates of all 12 sheep increased, blood oxygen saturation and partial pressure of oxygen i
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2010年第5期363-366,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
肺栓塞
模型
动物
血栓
Pulmonary embolism
Blood clot,Balloon Catheterization,Animal model
