摘要
本文应用组织抗原微波修复的SP免疫组化染色法研究了22例原发性肝癌(PHC)、10例肝内海棉状血管瘤、11例肝硬化及11例正常肝组织中P糖蛋白(PqP)的表达。22例PHC中有19例(86%)不同程度地表达了P糖蛋白阳性。11例肝硬化中5例阳性,10例海棉状血管瘤全呈阴性,11例正常肝细胞呈低到中度阳性。肝内不同病变中PqP的染色部位和强度有所不同:肝癌细胞的阳性染色主要位于细胞膜上,混合性肝癌的腺腔内膜呈阳性,肝硬化组织以细胞浆染色为主,而正常肝细胞的染色位于细胞浆内,膜无明显着色。本文结果提示PqP在肝脏病变中的表达不仅能从分子水平上客观判断肿瘤细胞的化疗敏感性、指导化疗用药而且PqP表达的部位和强度可能与肝癌的恶性进程有关。
Using S P Immunohistochemical methods with micro ray restoring tissue antigen,we examined the expression of P qP in 22 primary hepatocarcinoma(PHC),10 hepatic spongyvascular tumors,11 cirrhosis and 11 normal hepatic tissues.Among samples analysized,P qP positive was detected in 19(86%) of 22 PHC,5 of 11 in cirrhosis;P qP showed minimal and mid positive in 11 normal tissues,while P qP dyeing in hepatic pathological lesions were different:positive granute of hepatocellular carcinoma(HCC) is mainly in cell menbrane;In M PHC,Gland cavity endo menbrance was positive;Cell menbrance,cytoplasm of cirrhosis were mini mid positive;While location of positive granule is cytoplasm in normal hepatic cells,no positive granule in cell menbrance.These results show expression of P qP in hepatic pathological lesions not only can judge the sensitivity of chemotherapy for cancer patients,but also the location and intensity of P qP expression might relate with the development of hepatocarcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1999年第2期96-97,共2页
Cancer Research on Prevention and Treatment
关键词
P-糖蛋白
S-P
免疫组化
药物耐受
肝癌
肝硬化
P qP
hepatic pathological lesion
S P immunohistochemical method
drug resistance
