摘要
目的：研究神经元特异性烯醇化酶（neuronspecificenolase，NSE）的表达，与p53蛋白和增殖细胞核抗原（prolif－erating－cellnuclearantigen，PCNA）表达的关系及意义．方法：用特异性鼠抗人单克隆抗体，按LSAB免疫组织化学方法检测石蜡包埋的肺癌标本中NSE，p53蛋白和PCNA的表达．结果：在小细胞性肺癌（SmallCellLungCancer，SCLC）中，NSE阳性表达者，PCNA标记指数（LabellingIndex，LI）高于NSE阴性者，而p53蛋白的表达与NSE的表达无关．在非小细胞性肺癌（non－smallcelllungcancr，non－SCLC）中，p53蛋白的表达和PCNALI均与NSE的表达无关．结论：在SCLC的发生及发展过程中，神经内分泌功能可能起了部分作用．而p53抑癌基因在SCLC的发生中并不起着重要的作用．
AIM: To study the relationship between expression of neuron specific enolase (NSE) and p53 protein and labelling index (LI ) of proliferating-cell nuclear antigen (PCNA) and the significance of them in the progression of lung cancer. METHODS: The expression of p53 protein,NSE and PCNA was determined respectively by immunohistochemical technique with monoclonal antibodies. RESULTS:There is a statistical difference in PCNA LI between small cell lung cancer (SCLC) with NSE and SCLC without NSE.There is no relationship between the expression of p53 protein and NSE. In non-SCLC, the expression of both p53 protein and PCNA LI has no relation to the expression of NSE with monoclinal antibodies. CONCLUSION: Neuroendocrine plays a role in the progression of SCLC to some extent, whereas the mutation of p53 supressive gene is not implicative of the carcinogenesis of SCLC.
出处
《第四军医大学学报》
1999年第3期246-248,共3页
Journal of the Fourth Military Medical University
关键词
肺肿瘤
NSE
P53蛋白
PCNA
免疫组织化学
lung neoplasms
neuron specific enolase
proliferating-cell nuclear antigen
p53 protein
immunohistochemistry
