摘要
目的研究益气活血法对血瘀证缺血再灌注损伤(IRI)兔心肌eNOS蛋白表达的影响,以探讨其对心肌IRI保护作用机制。方法建立兔血瘀证IRI模型,以加味丹参饮对该模型进行干预,采用免疫组化法观察心肌eNOS蛋白表达。结果 IRI组心肌eNOS蛋白表达降低,与空白组比较差异具有统计学意义;加味丹参饮组可以提高eNOS蛋白表达,与IRI组比较差异具有统计学意义。结论益气活血法可以提高eNOS蛋白表达,其为保护心肌IRI作用机制之一。
Objective： To study the effect of Yiqihuoxue method on eNOS protein expression in myocardial ischemia reperfusion injury （IRI） rabbits with blood stasis syndrome and to detect the mechanism of this therapy in protecting IRI. Methods： The model of rabbit with blood stasis syndrome was intervened by the supplemented salvia decoction. The eNOS protein was detected by the immunohistochemical method. Results： eNOS protein expressions were decreased in the IRI group. There was a significant difference between this group and the control group. This expression which was obvious difference compared to the IRI group was increased by the supplemented salvia decoction. Conclusion： It could be the one of mechanisms in protecting myocardial muscle that the eNOS is improved by the Yiqihuoxue method.
出处
《中国中医急症》
2010年第6期993-995,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然基金项目(No.30973750)
湖南省自然科学基金项目(No.05JJ40025)
湖南省科技计划项目(No.2008FJ3070)
湖南省中医药科研计划项目(No.2008022)
关键词
益气活血法
血瘀证
心肌缺血再灌注损伤
ENOS
兔
Yiqihuoxue method
Blood stasis syndrome
Myocardial ischemia reperfusion injury
eNOS
Rabbits
