摘要
目的:研究热化疗对人小细胞肺癌H446细胞增殖的影响及其可能机制。方法:H446细胞分为单纯化疗组(单纯使用120μg/L紫杉醇处理细胞)、热化联合组(采用43℃加热联合120μg/L紫杉醇处理细胞)、抑制剂组(采用43℃加热联合120μg/L紫杉醇及1μmol/LAkt特异抑制剂wortmannin处理细胞),以未处理的H446细胞作对照。应用MTT法检测各组细胞增殖率的变化,采用WesternBlot检测各组细胞磷酸化Akt水平。结果:对照组、单纯化疗组、热化联合组和抑制剂组细胞增殖率分别为(100.00±0.00)%、(69.16±2.95)%,(59.83±3.36)%和(40.65±0.14)%,磷酸化AKt水平分别为(1.52±0.01),(1.04±0.42),(0.69±0.03)和(0.00±0.00),4组细胞增殖率及磷酸化Akt水平相比,差异均有统计学意义(F=68.231和6.232,P均<0.05)。与对照组和单纯化疗组相比,热化联合组细胞增殖率及磷酸化Akt水平均降低(P<0.05),wortmannin可进一步抑制H446细胞的磷酸化,且降低细胞的增殖率(P<0.05)。结论:热化疗联合应用可抑制H446细胞增殖,其作用可能是通过抑制Akt信号转导通路实现的。
Aim：To study the effect of thermo-chemotherapy on the proliferation of lung cancer H446 cells and its possible mechanism.Methods：H446 cells were allocated into 4 groups：cells in chemotherapy group were given 120 μg/L paclitaxel,those in thermo-chemotherapy group were treated by paclitaxel and heating at 43 ℃,those in inhibitor group were treated by Akt inhibitor,wortmannin,besides the treatment as thermo-chemotherapy group.The cells untreated served as control. MTT assay was used to measure cell proliferation,and Western Blot was used to examine the phosphorylation level of Akt.Results：The cell proliferation rates of the 4 groups were （100.00±0.00）%,（69.16±2.95）%,（59.83±3.36）%,and （40.65±0.14）%,and the phosphorylation level of Akt of the 4 groups were （1.52±0.01）,（1.04±0.42）,（0.69±0.03）,and （0.00±0.00）,and there were significant differences（F=68.231 and 6.232,P0.05）. Compared with control group and chemotherapy group,the cell proliferation rate and the phosphorylation level of Akt in the thermo-chemotherapy group were decreased（P0.05）,and wortmannin could further inhibit the phosphorylation of Akt and the proliferation rate（P0.05）.Conclusion：Thermo-chemotherapy can inhibit the proliferation of H446 cells,which may be realized by inhibiting Akt pathway.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2010年第3期375-378,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30571552
郑州市科技发展计划(攻关计划)基金资助项目19-153(2006)
关键词
H446细胞
化疗
热化疗
AKT
细胞增殖
H446 cell
chemotherapy
thermo-chemotherapy
Akt
cell proliferation
