摘要
目的 探讨地塞米松（DEX）对脑膜炎家兔脑脊液（CSF）中血管内皮生长因子（VEGF）质量浓度变化的影响,为临床应用DEX辅助治疗脑膜炎提供理论依据.方法 家兔36只,随机分为模型组、DEX处理组和对照组.于注射大肠埃希菌悬液后6、12及24 h采用双抗夹心ELISA法定量检测兔CSF中VEGF的质量浓度.结果 模型组6、12及24h CSF中VEGF质量浓度[（1 219±176）ng/L、（1 343±160）ng/L、（981±134）ng/L]明显高于同时相对照组[（374±172）ng/L、（370±169）ng/L、（367±171）ng/L],差异有非常显著性（P〈0.01）;脑组织含水量高于对照组[（80.8±0.5）%vs（80.0±0.5）%],差异有非常显著性（P〈0.01）;脑组织含水量与24 h CSF中VEGF质量浓度呈正相关（r=0.919,P〈0.01）.DEX处理组6、12及24 h CSF中VEGF质量浓度[（941±147）ng/L、（1 083±123）ng/L、（825±66）ng/L]低于同时相模型组,差异有显著性（P〈0.05）,脑组织含水量（80.4±0.5）%低于模型组,差异有显著性（P〈0.05）.结论 细菌性脑膜炎病程中VEGF分泌增多,VEGF参与脑水肿形成;DEX通过抑制VEGF表达和（或）分泌,减轻血脑屏障损害,抑制脑水肿形成.
Objective To investigate the effects of dexamethasone （DEX） on vascular endothelial growth factor（VEGF） in cerebrospinal fluid of rabbits with bacterial meningitis. Methods A total of 36 rabbits were assigned to study, which were randomly divided into meningitis model group （MOD）, dexametha- sone-treated group （DEXT） and control group（CON）. CSF was sampled for determining at 6 h, 12 h and 24 h after injection of E. coli suspension. The concentration of VEGF in every CSF sample was determined quantitatively by ELISA. Results There were higher concentrations of CSF VEGF at 6 h, 12 h and 24 h in MOD（ （ 1 219 ± 176） ng/L, （ 1 343 ± 160） ng/L, （981 ± 134） ng/L） than that in CON（ （374 ± 172） rig/L, （370 ± 169） ng/L, （367± 171 ） ng/L） （P 〈 0. 01 ）. There was higher brain water content in MOD （ （ 80. 8± 0. 5）% ） than that in CON（ （80. 0±0. 5）% ） （P 〈0.01 ）. There was positive correlation between the brain water content and the concentration of CSF VEGF at 24 h （ r = 0. 919, P 〈 0. 01 ）. Compared with MOD, the concentrations of CSF VEGF in DEXT at 6 h,12 h,24 h （ （941 ± 147） ng/L, （ 1083 ± 123） ng/L, （825 ± 66 ） ng/L） were decreased significantly（ P 〈 0. 05 ）, the brain water content was less （ （ 80.4 ± 0. 5 ） % ） （P 〈 0. 05）. Conclusion The secretion of VEGF markedly increases in the pathological process of bacterial meningitis. VEGF contributes to the damage of blood brain barrier and the formation of brain edema. DEX can decrease the degree of brain edema by suppressing the generation of VEGF and lightening the damage of blood brain barrier.
出处
《中国小儿急救医学》
CAS
2010年第3期242-243,247,共3页
Chinese Pediatric Emergency Medicine
关键词
血管内皮生长因子
脑膜炎
脑脊液
地塞米松
家兔
Vascular endothelial growth factor
Meningitis
Cerebrospinal fluid
Dexamethasone
Rabbit
