摘要
目的:探讨构建神经纤维束显微结构三维可视化模型的实用方法。方法:SD大鼠全脑连续冰冻切片经Luxol Fast Blue染色显示锥体束。采用分区显微摄影法,获取每张脑组织切片的二维图像。运用Photoshop 7.0软件对每张脑片的图像进行拼接,根据三轴定位法对拼接图像进行配准,导入3D-DOCTOR4.0软件对脑和锥体束进行表面重建和体素重建。利用3DMAX8.0软件对重建模型进行可视化处理。结果:重建出SD大鼠脑和锥体束全貌模型清晰逼真,可以在任意断面从不同角度观察锥体束的显微结构,动态显示锥体束在脑内的立体行径。结论:基于连续组织切片的三轴定位法结合三维重建软件可以真实地再现锥体束的显微结构及其在脑内的三维立体行径,为神经纤维束的损伤修复研究提供精确的三维立体形态学基础。
Objective：To explore the applicated methods for 3D visualization model of the microstructure of the rat neural fasciculus.Methods：The pyramidal tracts of the rat brain were revealed by the Luxol Fast Blue（LFB） staining method on the serial brain sections.The 2D images were obtained by the partitional microphotography on each brain section.The images of each brain section were stitched and registrated according to the three-axis location system in the Photoshop7.0 software.The registrated images were imported into the 3D-DOCTOR 4.0 reconstruction system to make the surface reconstruction and the volume reconstruction of the brain and the pyramidal tracts.The reconstructed pyramidal tract models were visulized in the 3DMAX8.0 software.Results：The whole shape of the brain and the pyramidal tract were reconstructed clearly and vividly,the reconstructed microstructure of the pyramidal tracts can been observed in different angles at any cross section,the 3D stereopathway of pyramidal tracts was dynamically displayed.Conclusion：These results show that the microstructure of pyramidal tract and its stereopathway in the brain can be actually and acurately reconstructed and re-appeared by the three axis location system based on the serial sections,thus provides an acurate 3D morphological base for the research on the neural foasciculus damage and repairing.
出处
《南通大学学报（医学版）》
2010年第1期17-20,F0002,共5页
Journal of Nantong University(Medical sciences)
基金
南通市"226高层次人才培养工程"项目资助计划(通委人才办[2006]16号)
关键词
连续切片
三维重建
锥体束
大鼠
Serial sections
Three dimensional reconstruction
Pyramidal tract
Rat
