摘要
从四川省3个蔬菜基地感病的甘蓝、花菜、萝卜叶芥菜分离到芜菁花叶病毒6个分离物.利用RT-PCR克隆了这6个分离物的外壳蛋白基因(CP),测定了它们的核苷酸序列并进行了序列分析.结果表明,6个分离物的CP基因均为864个碱基,核苷酸序列同源性较高,达97.0%～100%;它们与world-B组分离物的同源性最高,达92.1%～100%;与basal-B组分离物的同源性最低,仅87.6%～90.2%.基于TuMV的CP基因核苷酸序列的分子进化树显示,TuMV分离物可分为4组,本研究所分离到的6个TuMV四川分离物属于第四组,即world-B组.
Six isolates of turnip mosaic virus were isolated from infected Cabbage, Cauliflower, Radish, Leaf mustard in three different regions of Sichuan province. A pair of primers was designed and synthesized based on the nucleotide sequences of coat protein （CP） genes of TuMV reported. The CP genes of the six TuMV isolates were cloned by RT-PCR. All the CP genes from the six isolates were sequenced and compared with the CP gene sequences of TuMV reported in GenBank. The results showed that the sequences of the six CP genes were all 864 nucleotides in length, and the isolates shared high sequence homology from 97.0% to 100%. They also shared high homologies of 92. 1% to 100% with the sequences of world-B group isolates and of 87.6%~90.2% with the sequences of basal-B group isolates. TuMV isolates reported so far were divided into four groups based on the phylogenetic analysis of CP gene sequences. All the six isolates in this study belonged to the fourth group, i.e. world-B group.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期639-643,共5页
Journal of Sichuan University(Natural Science Edition)
基金
教育部博士点基金(20070610172)
四川省科技厅项目(07KJT-08和07JY029-068)
