摘要
根据GeneBank中提供的牛乳铁蛋白基因序列,设计并合成了重组LfcinB/LfampinB基因,并通过PGEM-Teasy载体扩增。酶切回收的LfcinB/LfampinB片段与中间载体pP6连接。pP6上的启动子、信号肽和调控序列与LfcinB/LfampinB共同切下,获得具有上游调控元件的PLfc/Lfa基因片段。将PLfc/Lfa与pME290表达载体连接,并转化绿脓杆菌。结果表明,本试验成功构建了重组pPLfc/Lfa表达载体。
the Bovine lactoferrin gene deposited in GeneBank,LfcinB/LfampinB recombinant gene was designed and synthesized,and then amplified by PGEM-T easy plasmid.The purified LfcinB/LfampinB gene by restriction enzyme was linked to pP6 plasmid.Together with LfcinB/LfampinB gene,the promoter,signal peptide and regulator gene which were in pP6 plasmid were digested by restriction enzyme,PLfc/Lfa fragment which had upstream regulatory element was obtained.PLfc/Lfa was linked to pME290 expression plasmid and transformed into competent cells of.The results showed that recombinant expression plasmid pPLfc/Lfa was obtained.
出处
《特产研究》
2010年第2期16-18,39,共4页
Special Wild Economic Animal and Plant Research
基金
中国农业科学院特产研究所基金项目
关键词
抗菌肽
克隆
表达载体
构建
Antimicrobial peptide
Clone
Expressional plasmid
Construction
