摘要
将经过２次新城疫（ＮＤ）基础免疫的ＳＰＦ鸡，随机分成５组，每组８只，在６０日龄时分别用Ｆ４８Ｅ８、Ｔｅｘａｓ、Ｈｅｒｔ３３ＮＤ强毒和Ｌａｓｏｔａ弱毒经口腔接种０．２５ｍＬ／只，１２ｈ后采取泄殖腔棉拭，利用ＮＤ强毒快速诊断试剂盒进行排毒的动态监测，并在攻毒前期、中期、后期测定各试验组ＳＰＦ鸡的ＨＩ效价。试验第３天开始排毒，２０ｄ结束，其间在６～７ｄ和１１～１３ｄ出现２次排毒高峰。ＨＩ抗体在初期先是降低１个滴度，后期开始升高而且离散度不断增大。
A ELISA kit based on monoclonal antibodies was used to detect the dynamical change of shedding virus in SPF chicken flocks infected with different pathotypes of NDV (F48E8, Hert33, Texas, Lasota) and HI levels at early, middle and late stage post inoculation were detected by standard method. The results showed that only chicken flocks infected with virulent strains excreted viruses begining at three days of inoculation and ending on twentieth day. There were two peaks of shedding virus in chicken flocks infected with virulent strains of NDV. One peak appeared in 5 6 days of inoculation and another peak in 11 13 days of inoculation. While the chicken flocks infected with commonly mesogenin and lentogenic vaccine strains did not shed virus from cloacal.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第1期15-17,共3页
Chinese Journal of Veterinary Science
基金
国家"九五"攻关
江苏省"九五"攻关课题
