摘要
为研究包埋硝化细菌驯化阶段的特征,以模拟氨氮废水为进水,按10%～15%的体积填充率,维持系统DO的质量浓度为3～5mg·L-1,在流化床反应器中进行连续式培养驯化。以每日进出水氨氮含量为监测指标,同时进行聚合酶链式(PCR)扩增试验,考察包埋硝化菌颗粒活性与颗粒内细菌数量变化情况。结果表明,驯化后硝化细菌活性有了很大提高,载体颗粒内目标细菌氨氧化菌得到有效富集,包埋颗粒活性由10.873上升至234.46mg·L-1.h-1;在进水氨氮的质量浓度360mg·L-1时,驯化结束时的氨氮去除率稳定在90%以上;载体颗粒内的生物含量明显增加。
In order to study the characteristics of immobilized bacteria in the acclimation period,a continuous experiment was conducted. The pellets stuffing ratio in volume was kept between 10%～15%. The synthetic ammonia-rich wastewater was used as the influent water and DO was maintained at 3～5 mg·L^-1. The influent and effluent NH4^＋-N concentration and the pellet＇s oxygen consumption rate were monitored and PCR was also carried out to investigate the change of the ammonium-oxidizing bacteria. The results showed that the nitro-bacterial degradability was enhanced considerably and the quantity of the ammonia oxidation bacteria increased substantially during the acclimation. At the end of the process,the pellet＇s oxygen consuming ability increased from 21.045 to 227.806 mg·L^-1·h^-1 and the removal rate remained steadily above 90% at the influent NH4^＋-N concentration of 360 mg·L^-1.
出处
《水处理技术》
CAS
CSCD
北大核心
2010年第6期73-76,共4页
Technology of Water Treatment
基金
国家水体污染控制与治理科技重大专项(2008ZX07317-006)
浙江省科技计划项目(2008C23092)
关键词
包埋固定化
硝化菌
驯化
氨氧化菌
PCR扩增
immobilized nitrobacteria
acclimation
ammonia oxidation bacteria
PCR experiment
