摘要
探讨聚腺苷二磷酸核糖聚合酶-1(PARP1)Val762 Ala,Lys 940Arg基因多态性与甘肃省地区汉族人群胃癌易感性的关系.方法:采用多重单碱基延伸SNP分型技术(Multiplex SNaPshot)对甘肃省胃癌高发区河西地区汉族人群中经病理确诊的原发性胃癌150例,健康对照组152例进行PARP-1基因2个SNP位点基因分型.使用ELISA法检测H.pylori感染.结果:发现PARP-1940Lys/Arg基因型分布在胃癌组明显高于对照组(OR=2.917,95%CI1.430-5.947,P=0.002);PARP-1762Val/Al基因型分布在胃癌组明显高于对照组(OR=1.685,95%CI:1.040-2.729,P=0.034).分层分析显示,在吸烟人群中,PARP-1940 Lys/Arg基因型携带者患胃癌的风险是Lys/Lys型携带者的8.430倍(OR=8.340;95%CI:2.664-26.144P=0.000);在饮酒人群中,PARP-1940LysA r g基因型携带者患胃癌的风险是Ly s/Ly型携带者的3.333倍(OR=3.333,95%CI1.214-9.155,P=0.015),在H.pylori感染阳性人群中,PARP-1762Ala/Ala基因型携带者患胃癌的风险是Val/Val携带者的2.360倍(OR=2.360,95%CI:1.256-4.433,P=0.007).同时携带PARP-1940Lys/Arg和PARP-1762AlaAla+Val/Ala基因型的个体患胃癌的发病风险是PARP-1940Lys/Lys和PARP-1762Val/Va基因型携带者的4.2倍(OR=4.200,95%CI1.430-12.338,P=0.006).结论:PARP-1 940Lys/Arg和PARP-1 762AlaAla或Ala/Ala基因型与中国甘肃地区汉族人群胃癌发病风险增高相关;PARP-1Lys940Ar与吸烟,饮酒,PARP-1Val762Ala与H.pylor感染以及PARP-1Lys940Arg与PARP-Val762Ala在胃癌的发病风险中都各自存在着加乘交互效应.
AIM：To investigate the association between the Val762Ala and Lys940Arg polymorphisms of the poly（ADP-ribose） polymerase-1（PARP-1） gene and susceptibility to gastric cancer in a Chinese Han population in He＇xi area of Gansu Province.METHODS：All investigated subjects were divided into two groups：150 gastric cancer patients and 152 controls.The SNaPshot single nucleotide polymorphism（SNP） genotyping method was used to analyze the genotypes of PARP-1 Val762Ala and Lys940Arg.Helicobacter pylori（H.pylori） IgG antibody was detected by enzyme-linked immunosorbent assay（ELISA）.The results were analyzed using the SPSS16.0 software package.RESULTS：PARP-1 940 Lys/Arg and 762 Val/Ala genotypes were overrepresented in gastric cancer patients compared with controls（OR=2.917 and 1.685;95%CI：1.430-5.947 and 1.040-2.729;P=0.002 and 0.034,respectively）.In smoking subjects,the risk of gastric cancer in PARP-1 940 Lys/Arg genotype carriers was 8.430-fold higher than that in Lys/Lys carriers（OR=8.340,95%CI：2.664-26.144,P=0.000）.In drinking subjects,PARP-1 940 Lys/Arg genotype carriers had a 3.333-fold higher risk of gastric cancer than Lys/Lys carriers（OR=3.333,95%CI：1.214-9.155,P=0.015）.In H.pylori IgGpositive subjects,PARP-1 762 Ala/Ala genotype carriers had a 2.360-fold increased risk of gastric cancer than Val/Val carriers（OR=2.360,95%CI：1.256-4.433,P=0.007）.The subjects carrying PARP-1 940 Lys/Arg and PARP-1 Ala/Ala or Val/Ala genotypes had a 4.2-fold increased risk of gastric cancer compared with those carrying PARP-1 940 Lys/Lys and PARP-1 762Val/Val genotypes（OR=4.200,95%CI：1.430-12.338,P=0.006）.CONCLUSION：PARP-1 940Lys/Arg and PARP-1 762Ala/Ala or Ala/Ala genotypes are associated with a higher risk of gastric cancer.There are multiplicative joint effects between PARP-1 940 Lys/Arg genotype and smoking or drinking,between PARP-1 762 Val/Ala or Ala/Ala genotypes and H.pylori infection,and between PARP-1 Lys940Arg and PARP-1 Val762Ala genotypes in increasing the risk
出处
《世界华人消化杂志》
CAS
北大核心
2010年第14期1434-1441,共8页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30870364~~
