摘要
通过L9(34)正交试验,研究不同根瘤菌梯度、接种方式和氮肥梯度对岷山红三叶Trifolium pratense异黄酮含量和产量的影响。结果表明,接种根瘤菌,对岷山红三叶异黄酮含量和产量都有显著影响(P<0.05),以每千克岷山红三叶种子接种16 g根瘤菌效果较好。接种根瘤菌方式、氮肥梯度对岷山红三叶异黄酮含量和产量不存在显著影响,但以拌种和不施氮肥效果较好。综合根瘤菌梯度、接种方式和氮肥梯度对岷山红三叶异黄酮含量和产量影响以每千克岷山红三叶种子与16 g根瘤菌肥拌种,不施氮肥效果最优。
The influence of rhizobia dose,inoculation methods and nitrogen fertilizer dose on the content of isoflavonoides in Trifolium pretense cv.Mingshan was study in an orthogonal experiment with three factors（3^4）.The results showed that inoculation rhizobia affected the total content and output of per unit area of isoflavonoides significantly（P〈0.05）,in which 16g rhizobia per kilogram seeds had the best effect.Seed treatment and no-nitrogen fertilizer treatment were better than others,thought no significant differences were found between inoculation methods and nitrogen fertilizer tests.In this study,inoculating rhizobia in 2 cm soil depth without fertilizer N seems to be helpful for increasing the production of isoflavonoides from the red clover.
出处
《草业科学》
CAS
CSCD
北大核心
2010年第5期97-100,共4页
Pratacultural Science
基金
岷山红三叶异黄酮含量动态变化及其调节动物钙磷代谢的作用及机理研究(GNMKJ-2009-01)
关键词
根瘤菌
施肥
岷山红三叶
异黄酮
Rhizobia
fertilization
Minshan red clover
Isoflavone
