摘要
建立了一种双手性拆分试剂（羟丙基-β-环糊精和羧甲基-β-环糊精）拆分烯丙洛尔对映体的毛细管电泳方法。烯丙洛尔对映体在pH 3.8的80 mmol/L Tris-H3PO4＋15 mmol/L羟丙基-β-环糊精（HP--βCD）＋5.0 g/L羧甲基-β-环糊精（CM--βCD）条件下获得成功分离。以（1R,2R）-2-氨基-1-苯基-1,3-丙二醇为内标,采用内标法定量,烯丙洛尔对映体在0.25-50 mg/L范围内具有良好线性关系,检出限（S/N=3）为0.25 mg/L,日内、日间RSD均不大于6%,平均回收率为97%-102%。该方法可用于鼠体内烯丙洛尔的药代动力学研究。
A capillary electrophoresis method was developed for the chiral separation of alprenolol using dual cyclodextrins（CDs） of hydroxypropyl-β-CD（HP-β-CD）and carboxymethyl-β-CD（CM-β-CD） as chiral selectors.The optimal separation conditions were optimized.A good baseline was obtained by using a running buffer of 80 mmol/L Tris-H3PO4 containing 15 mmol/L HP-β-CD and 5.0 g/L CM-β-CD at pH 3.8.The quantitative analysis of alprenolol enantiomer was performed by using（1R,2R）-2-amino-1-phenyl-1,3-propanediol（Pro）as an internal standard.Under the optimal conditions,the calibration curves were linear in the range of 0.25-50 mg/L for alprenolol enantiomer and the detection limit（S/N=3） were both 0.25 mg/L.Intra-and inter-RSDs were not more than 6%.Mean recoveries were in the range of 97%-102%.The proposed method could be used in pharmacokinetics studies.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第5期488-492,共5页
Journal of Instrumental Analysis
基金
广西自然科学基金资助项目(0640038)
肇庆市科技计划资助项目(10560)
肇庆学院自然科学基金资助项目(0737)
关键词
毛细管电泳
鼠血
烯丙洛尔
手性拆分
药代动力学研究
capillary electrophoresis
rat serum
alprenolol
chiral separation
pharmacokinetics studies
