摘要
在低浓度氨氮条件下利用厌氧折流板反应器(anaerobic baffled reactor,ABR)以厌氧污泥混合河涌底泥为接种源启动厌氧氨氧化(anaerobic ammonium oxidation,anammox)反应。系统连续运行4个月后出水趋于稳定,当NH3-N和NO2--N容积负荷分别为3.91 g/(m3.d)和3.21 g/(m3.d)时,平均去除率分别为85.7%和98.8%。利用荧光原位杂交(fluorescence in situ hybridization,FISH)技术对ABR厌氧污泥进行了分析。随后,以缩短HRT或增加进水NH3-N和NO2?-N浓度的方式来逐步提高反应器运行负荷,最后当NH3-N和NO2--N容积负荷分别达到65.53 g/(m3.d)和68.46 g/(m3.d)时,平均去除率为76.3%和91.3%,并培育出粒径为1.7～2.5 mm的颗粒污泥。利用扫描电镜(SEM)观察培育得到颗粒污泥与接种颗粒污泥,发现经驯化的ABR系统内微生物种类已变得较为单一,优势菌群发生明显变化。
Anaerobic ammonium oxidation（anammox） process was start-up in a anaerobic baffled reactor（ABR） inoculated with anaerobic sludge and river sludge under low ammonia concentrations.The water quality of effluent was stepwise reachered to a stable level after 4 months＇ enrichment operation.The average removal efficiencies of NH3-N and NO-2-N were 85.7% and 98.8% when the volume loading rate was 3.91 g/（m3·d） and 3.21 g/（m3·d） respectively.The anaerobic sludge in ABR was analyzed by fluorescence in situ hybridization（FISH）.Then the loading rate of this ABR system was increased gradually by shortening the HRT or by increasing NH3-N and NO-2-N concentrations in the influent.Finally,when the volume loading rate of NH3-N and NO-2-N were 65.53 g/（m3·d） and 68.46 g/（m3·d）,the average removal efficiencies reached 76.3%and 91.3%,respectively.Sludge granules with the diameter of 1.7～2.5 mm were developed.The enriched granular sludge and the initial seed granular sludge were obseverd by scan electronic microscope（SEM）,the microorganism species in the enriched granular sludge with high anammox activity became much simplified comparing to those of the initial seed granule.
出处
《环境工程学报》
CAS
CSCD
北大核心
2010年第6期1224-1230,共7页
Chinese Journal of Environmental Engineering
基金
国家高技术研究发展计划(863)项目(2006AA06Z322)
广东省科学院野外科学实验站基金项目(sytz2008)
