摘要
本文采用显微荧光定量和生物物理等技术方法,应用西德 OPTON 公司制造的 SMP-05型紫外显微分光光度计,对男性不育症患者和正常有生育力成年男性精子膜表面麦胚凝集素(Wheat germ agglutinin,缩写 WGA)受体和精子膜蛋白质大分子对(I-Anilinonaph thalene-8-Suphonic acid salt,简称1,8-ANS)的荧光偏振度(P)及荧光强度(Ⅰ)进行了定量研究,结果发现:患者精子膜表面麦胚凝集素受体低下(P<0.001),精子膜蛋白质大分子疏水区1,8-ANS 荧光强度过强(P<0.05)。经中药生精种子汤治愈后,精子膜表面 WGA 受体明显增加,1,8-ANS 荧光强度接近正常。提示:中药对精子质膜 WGA受体及质膜蛋白质大分子结构有一定影响。
The authors carried on the quantitative analysis of fluorescence polarization degree and fluores- cent intensity on wheat germ agglutinin(WGA)receptor in sperm membrane surface and protein macromolecule 1-Anilinonaph thalene-8-suphonic acid salt(1,8-ANS)of sperm membrane to the patients with infertility and adult male with normal fertility,with the method of ultraviolet microspectrofluorometer(SMP,type:05,made in West Germany).The results were as follows:the patient's WGA receptor in sperm membrane surface was decreased(P<0.001),and the 1,8-ANS of fluorescent intensity in hydrophobic area of protein macromolecule of sperm membrane was in- creased(P<0.05).After the treatment of Shenjing,Zhongzitang(生精种子汤),WGA receptor was increased obviously and 1,8-ANS of fluorescent intensity was changed to be almost normal.It showed that the Chinese medicinal herbs mentioned above have certain influence upon the constitu- tion of WGA receptor and protein macromolecule of sperm membrane.
